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Heart-type fatty acid binding protein detection kit and making method thereof

A fatty acid combination and protein detection technology, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of narrow detection linear range, poor reagent stability, poor anti-interference ability, etc., and achieve wide detection linear range, Accelerate the speed of immune response and improve the effect of stability

Active Publication Date: 2014-12-17
上海睿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the latex immunoturbidimetric method has great research value, but the latex-enhanced immunoturbidimetric assay kits currently on the market have problems such as poor anti-interference ability, low specificity, poor reagent stability or narrow detection linear range. Room for improvement

Method used

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  • Heart-type fatty acid binding protein detection kit and making method thereof
  • Heart-type fatty acid binding protein detection kit and making method thereof
  • Heart-type fatty acid binding protein detection kit and making method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This embodiment relates to the preparation of a H-FABP detection kit, including components and contents as follows:

[0054] Reagent R1:

[0055] components

specific type

Content description

Buffer solution with pH=7.0~7.6

Glycine buffer

120mmol / L, pH7.4

Surfactant

Tween 20

10ml / L

Chelating agent

Disodium edetate

50mmol / L

Accelerator

Dextran 8000

200mmol / L

preservatives

NaN 3

1‰

[0056] Various ingredients can be added sequentially at room temperature, sealed and stored for later use. Alternatively, they are packaged individually and prepared immediately prior to testing.

[0057] Reagent R2: The preparation of latex reagent of anti-human H-FABP antibody includes the following steps.

[0058] Step 1: Acidifying the mouse anti-human H-FABP antibody in MES solution with a pH value of 4.0-6.0;

[0059] Step 2: Adjust the pH of the solution obtained in Step 1 to 7.4-8.2...

Embodiment 2

[0067] The H-FABP detection kit described in this example is applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: two-point endpoint method, that is, the dosage of reagent R1 and reagent R2 is 150ul and 50ul respectively, and the sample volume is 10ul.

[0068] Adopt kit reagent of the present invention and above-mentioned assay method, adopt the curve of the H-FABP standard substance (self-made) of 6 kinds of different contents that Hitachi 7170 biochemical analyzer records (such as figure 1 Shown), each point represents a content of the reference standard, wherein the X-axis represents the H-FABP content (ng / ml); Y-axis represents the absorbance.

[0069] Table 1

[0070]

Embodiment 3

[0071] Embodiment 3: Correlation test of detection reagent

[0072] Use the reagent of this method invention (specific formula is the same as embodiment 1) and the H-FABP latex enhanced immune turbidimetric reagent of British Landau Company of contrast reagent, adopt automatic 7170 full-automatic biochemical analyzers to 50 parts of human serum (normal and abnormal specimens) ) were measured simultaneously according to their respective parameters, and correlation analysis was carried out on the measured values.

[0073] See the test results figure 2 , X, Y axis are measured value (the content ng / ml of H-FABP), by figure 2 The results show that the correlation between the two reagents is R 2 =0.9947, the regression equation is y=0.9854x+0.3527. The result shows that the kit reagent of the present invention has good correlation with British Landau's determination of patient's serum, and has good specificity and accuracy.

[0074] In addition, the above experiments were c...

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Abstract

The invention relates to a heart-type fatty acid binding protein (H-FABP) detection kit and a making method thereof. The kit comprises a reagent R1, a reagent R2 and a standard substance; the reagent R1 comprises a solvent and solvates, the solvent is a buffer solution with the pH value of 7.0-7.6, and the solvates comprise a surfactant, a chelating agent, an accelerator, an antiseptic and purified water; the reagent R2 is an anti-human H-FABP antibody latex reagent; and the standard substance is a recombinant protein containing a quantitative amount of the H-FABP. The problems of bad anti-interference capability, low specificity, low reagent stability and narrow detection linearity range of latex enhanced immunoturbidimetery detection kits in the present market are solved. The kit disclosed in the invention has the characteristics of simple operation, high accuracy, good repeatability, high sensitivity and the like, can be used on a fully-automatic biochemical analyzer, a special protein analyzer or a spectrophotometer, and has a wide application prospect.

Description

technical field [0001] The invention relates to a detection kit for cardiac fatty acid binding protein and a preparation method thereof, in particular to a detection kit for improving the detection sensitivity of cardiac fatty acid binding protein by using latex-enhanced immune turbidimetry technology. Background technique [0002] Acute myocardial infarction (AMI) is one of the diseases that seriously threaten human health. In recent years, its morbidity and mortality have been increasing year by year, and there is a trend of getting younger and younger. According to statistics, more than 1 million people die from AMI every year in China. According to modern therapeutics, rapid diagnosis and reperfusion therapy after the onset of AMI are very important for reducing mortality and improving prognosis. [0003] Hospitalized patients are usually tested for at least 6 to 12 hours before being considered for discharge before acute myocardial infarction (AMI) is completely ruled ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6887
Inventor 李伟奇赵小凤房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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