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Method and device for immunological detection

An immunological detection method and immunological detection technology, which are applied in measurement devices, biological tests, material inspection products, etc., can solve problems such as poor detection sensitivity, and achieve the effects of improving detection sensitivity and increasing impedance changes.

Inactive Publication Date: 2011-06-15
杭州安远科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In immune reactions, the use of solution impedance for measurement has the main disadvantage of poor detection sensitivity

Method used

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  • Method and device for immunological detection
  • Method and device for immunological detection

Examples

Experimental program
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Embodiment 1

[0049] An immunoassay device for detecting Salmonella and Escherichia coli E coliO157: H7, said device comprising:

[0050] Provide a 50x50 microelectrode array, and the distance between two adjacent electrodes is 5 microns;

[0051] Primary antibodies against Salmonella and E. coli E coliO157:H7; applied to the front of the microelectrode array;

[0052] A micromagnetic ball of 1 micron, with a secondary antibody corresponding to the above-mentioned primary antibody on the micromagnetic ball;

[0053] The magnet, when the magnet is at the back of the microelectrode array, helps the micromagnetic balls to be adsorbed on the surface of the microelectrode array; when the magnet is at the front of the microelectrode array, it helps to suck away the unresponsive micromagnetic balls.

[0054] A module for washing the array surface;

[0055] Buffer; this embodiment uses a low ionic strength phosphate buffer.

[0056] A measurement module for measuring parameters between the micro...

Embodiment 2

[0066] An immunoassay device for detecting Salmonella and Escherichia coli E coliO157: H7, the difference from Example 1 is:

[0067] 1. The distance between each pair of electrodes is 1 micron.

[0068] An immunoassay method for detecting Salmonella and Escherichia coli E coliO157: H7, the difference from Example 1 is:

[0069] 1. The distance between each pair of electrodes is 1 micron.

Embodiment 3

[0071] An immunoassay device for detecting Salmonella and Escherichia coli E coliO157: H7, the difference from Example 1 is:

[0072] 1. The distance between each pair of electrodes is 1 micron.

[0073] 2. Use 1nm magnetic balls.

[0074] An immunoassay method for detecting Salmonella and Escherichia coli E coliO157: H7, the difference from Example 1 is:

[0075] 1. The distance between each pair of electrodes is 1 micron.

[0076] 2. Use 1nm magnetic balls.

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Abstract

The invention relates to an immunological detection method which comprises the following steps of: providing an array which comprises at least two pairs of microelectrodes; arranging a first anti-antibody corresponding to a detected component on the surface of the array; additionally arranging a detected object and a micro magnetic ball on the surface of the array; arranging a second anti-antibody corresponding to the first anti-antibody on the micro magnetic ball; cleaning the surface of the array with a cleaning plate; and adding a buffer solution on the surface of the array, measuring the parameters among the microelectrodes, and analyzing the parameters to obtain the content of the detected component in the detected object. The invention also discloses a device for realizing the immunological detection method. The device and the method for immunological detection, disclosed by the invention, have the advantages of simultaneous detection of multiple components, high detection sensitivity, simple detection device and the like.

Description

technical field [0001] The invention relates to an immunological detection technology, in particular adopting an impedance array chip to perform multi-component detection at the same time, using micro magnetic balls to amplify signals, and using an external magnetic field to remove the magnetic balls to reduce the background signal. Background technique [0002] Enzyme-linked immunoassay (ELISA) is a common immunological method, which uses a primary antibody to capture the target to be tested, and uses an enzyme-labeled secondary antibody to achieve signal detection. Due to the catalytic amplification of enzymes, ELISA can achieve more sensitive detection. However, enzymes are biological substances, and their performance is affected by many factors, so their stability is not very good. At the same time, at present, people mainly use some optical means, such as colorimetry, fluorescence method, etc. to detect the catalytic substrate or product of the enzyme. Although optical...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/569G01N33/68
Inventor 吴坚
Owner 杭州安远科技有限公司
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