Troponin-T determination kit

Abstract

The invention relates to a kit for determining troponin-T (TNT) content of serum, aims to overcome the deficiencies of the above background technology, and provides a kit for detecting troponin-T by an immunoturbidimetry method, wherein the kit does not require sample dilution, has simple operation, high accuracy and good repeatability, and is suitable for various types of fully automatic biochemical analyzers and special protein instruments. A technical scheme is as below: the kit comprises: A. a reagent R1 containing a buffer solution, a preservative, an accelerating agent, inorganic salts, a surfactant and the balance of purified water; b. a reagent R2 containing a buffer solution, latex microspheres with diameter of 60-150 nm combined with anti-human troponin antibodies and an antiseptic; and c. a reference calibrator containing a buffer solution, a stabilizer, a preservative, a certain amount of recombinant human protein troponin-T pure product determined by concentration requirements and the balance of purified water.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a kit for measuring troponin content in human serum by adopting a latex-enhanced immune turbidimetric method. Background technique [0002] cTnT is a biochemical indicator with high sensitivity and specificity to reflect myocardial cell injury. The sensitivity of cTnT detection was 87.5%, the negative predictive value was 92.9%, and the accuracy was 66.7%. It has high sensitivity and specificity for the diagnosis of AMI. Some scholars also believe that the markers of myocardial injury cTnT and cTnI are the "gold standard" for the diagnosis of AMI. The window period of cTnT diagnosis of AMI is 3 hours to 21 days, which is more suitable for the diagnosis of AMI cases whose onset is more than 3 days and the electrocardiogram cannot confirm the diagnosis. Msir et al. found that the positive rates of cTnT, cTnI and CK-MB (mass analysis) for 4-hour diagnosis of AMI were 55%, 59%, and 6...

AI Technical Summary

Problems solved by technology

[0003] Currently clinically used TNT detection methods include: radioimmunoassay (IRA), which can directly measure blood plasma, but this method has environmental pollution and certain radioactive radiation to the operator; The method is more sensitive and accurate, but the cost is relatively expensive, and it needs supporting equipment before it can be used; the enzyme immunoassay (ELISA) has the disadvantages of cumbersome operation, sample pretreatment, and long detection time.

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