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Amylopsin activation detecting liquid and uses thereof

A technology for activity detection and pancreatic amylase, which is applied in the field of pancreatic amylase activity detection liquid, can solve problems that have not been done before, and achieve the effects of improving early detection rate, good response and connection, and reducing misdiagnosis rate

Inactive Publication Date: 2004-06-02
赵青华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, for the detection of pancreatic function, there have been no relevant reports on methods and products suitable for non-clinical "folk use"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of pancreatic amylase detection solution

[0027] In this example, 40 ml of a 25-fold concentrate was prepared. All operations are carried out in a sterile room, and the utensils have been sterilized in advance.

[0028] Weigh 3.4g of refined iodine, dissolve it in 2ml of distilled water, and stir to dissolve. Then, 6.8 g of potassium iodide was weighed, added into the obtained solution, and shaken until completely dissolved. Then add 10ml of glycerin and shake evenly. Transfer the resulting solution to a volumetric flask, and dilute to 40ml with distilled water, and shake evenly.

[0029] The obtained product was a brown solution with a pH of 4.85±1.2 and an absorbance of 2.0-2.5A at 480 nm. Store in a brown bottle away from light until use.

Embodiment 2

[0031] Sensitivity of Pancreatic Amylase Activity Detection Solution

[0032] Weigh 1g of soluble starch and 1g of sodium chloride, and make a paste with a small amount of distilled water. Pour the resulting paste into 90ml of boiling water, stir and boil for 3 minutes until clarified. Remove the heat source, wait until the boiling point of chloroform is below 60°C, add 3 drops of chloroform dropwise while it is warm, and mix well. Then make up to 100ml with distilled water. The resulting 1 w / v% starch solution was diluted with distilled water to prepare a 0.02 w / v% dilution. Then, prepare 0.01w / v%, 0.005w / v%, 0.0025w / v% and 0.00125w / v% starch solutions according to 2-fold serial dilution, each take 1ml and add to each transparent test tube, and indicate the concentration.

[0033] Starch solution (1ml)

[0034] The above tests show that the sensitivity of the detection solution of the present invention can reach 0.0025w / v% soluble starch conc...

Embodiment 3

[0036] Pancreatic function test

[0037] Element

[0038] Urine samples were obtained from patients with pancreatic fibrosis, normal subjects and patients with pancreatitis. It is known that patients with pancreatic fibrosis exhibit hypopancreatic function, while patients with pancreatitis exhibit hyperpancreatic function.

[0039] Temperature unit (U)

[0040] In short, take an 8-unit test tube as an example, add more than 10 drops of freshly prepared starch solution into the test tube with a 20±2 drops / ml dropper, then add 5 drops of urine specimen, and mark it as an 8-unit tube . By analogy, prepare 64-unit and 128-unit tubes, the difference is that only 1 drop of urine specimen is added dropwise.

[0041] At room temperature, cover the test tube with your hands and keep it for about 5 minutes;

[0042] Temperature unit (U)

[0043] The above results show that the trypsin function predicted by the color developme...

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PUM

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Abstract

The present invention provides one kind of amylopsin activity detecting solution and its use. The present invention makes it possible to realize the routine monitoring of pancreas function and the clinical detection of acute pancreatitis.

Description

technical field [0001] The invention relates to a liquid for detecting pancreatic amylase activity and its application. Background technique [0002] Acute pancreatitis is a sudden, high-mortality disease. Clinically, based on the severe abdominal pain at the onset, the misdiagnosis rate is high. Therefore, pancreatic function testing is needed for clinical diagnosis. Since pancreatic amylase is the most important digestive enzyme of the pancreas, the pancreatic function is generally judged clinically based on the detection of the activity of pancreatic amylase. For the detection of pancreatic amylase activity, Wen's method is the most commonly used at present (see "Clinical Medical Testing", the first edition in April 1978, Shanghai Science and Technology Press), but, because of its cumbersome operation, it has not been used clinically. , especially as a preventive diagnosis. [0003] On the other hand, with the continuous improvement of pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/40G01N33/50
Inventor 赵青华
Owner 赵青华
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