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A Method for Establishing a Deficiency Syndrome Tumor Cachexia Model

A method of establishing a cachexia technology, which is applied in the field of establishment of tumor cachexia models of deficiency syndromes, and can solve problems such as difficult to achieve treatment goals

Active Publication Date: 2021-08-24
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results of clinical application show that these methods are still difficult to achieve very effective therapeutic purposes

Method used

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  • A Method for Establishing a Deficiency Syndrome Tumor Cachexia Model
  • A Method for Establishing a Deficiency Syndrome Tumor Cachexia Model
  • A Method for Establishing a Deficiency Syndrome Tumor Cachexia Model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Establishment of Deficiency Syndrome Tumor Cachexia Model, etc.

[0068] Animal grouping: 8-week-old, healthy male BALB / c mice with a body weight of 24 ± 2 g were purchased from Shanghai Xipuer-Bikay Experimental Animal Company, and were randomly divided into 4 groups: blank control group (8 mice), asthenia syndrome group (8 mice) ), tumor cachexia group (8 rats), deficiency syndrome tumor cachexia group (8 rats).

[0069] Blank control group, normal feeding for 26 days;

[0070] Deficiency syndrome group, the modeling method is the same as the deficiency syndrome group in the preliminary embodiment 1, induced for 26 days;

[0071] In the tumor cachexia group, after 10 days of normal feeding, the mice were inoculated with C26 colon cancer cell suspension in the right anterior armpit and then fed for 16 days, the inoculation dose was 0.1 mL / mouse, and the inoculation process was completed within 1 hour;

[0072] Deficiency syndrome tumor cachexia group, the m...

Embodiment 2

[0085] Embodiment 2 adopts the drug screening method of deficiency syndrome tumor cachexia model

[0086] Animal grouping: 8-week-old, healthy male BALB / c mice with a weight of 24±2g were purchased from Shanghai Xipuer-Bikay Experimental Animal Company, and were randomly divided into 3 groups: blank control group (8 mice), deficiency syndrome tumor cachexia group (8 rats), deficiency syndrome tumor cachexia combined with drug (pyrrolidine dithiocarbamate, referred to as PDTC) treatment group (8 rats).

[0087] Blank control group, normal feeding for 26 days;

[0088] Deficiency syndrome tumor cachexia group is the same as the deficiency syndrome tumor cachexia group in Example 1, induced for 26 days;

[0089] Deficiency syndrome tumor cachexia combined with PDTC treatment group, its modeling method is the same as the deficiency syndrome tumor cachexia group, the only difference is that PDTC 100mg / kg is given intraperitoneally every day starting from the 11th day.

[0090] Ex...

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Abstract

The invention provides a method for establishing a tumor cachexia model of deficiency syndrome. The method comprises, at the same time, adopting the three-factor induction method of bitter cold diarrhea, hunger and fullness, and exhausted exercise to establish a deficiency syndrome mouse model, and inducing it for 7-15 days; inoculating the obtained deficiency syndrome mice with C26 colon adenocarcinoma cells to obtain deficiency syndrome Tumor cachexia model: After inoculation, the two-factor induction method of bitter cold diarrhea and abnormal hunger and fullness is adopted at the same time, and the induction is continued for 15‑18 days. Among them, body weight, thymus weight, spleen weight, and gastrin content were selected as observation indicators for deficiency syndrome. The animal model established by the above method can be used to study deficiency syndrome tumor cachexia or to screen drugs for treating deficiency syndrome tumor cachexia.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and detection, and specifically relates to a method for establishing a tumor cachexia model of deficiency syndrome. The model established by the establishment method can be used for studying cachexia of deficiency syndrome or screening drugs for treating cachexia of deficiency syndrome. Background technique [0002] Malignant tumors have become the most serious diseases that endanger human physical and mental health and are on the rise. Whether in developed or developing countries, malignant tumors are one of the top three causes of death for people over the age of 5. One of the important reasons affecting the survival of cancer patients is that most cancer patients develop cancer cachexia in the later stages of the disease course. Cachexia, also known as wasting syndrome, is a severe metabolic disorder in the body, mainly manifested as loss of muscle and fat accompanied by weight loss,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K36/482A01K67/027
CPCA01K67/027A61K35/13A61K36/482A61K49/0008
Inventor 刘璇李娜章雄文张周张万里苗春晓冯利兴
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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