Silage paper mulberry leaf feed microbial inoculum and application method thereof

A technology for constructing leaves and bacterial agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc., can solve problems such as the degradation of unpublished tannin substances, and achieve sustainable health promotion Effects on development, rapid growth, inhibition of growth and reproduction

Pending Publication Date: 2019-03-29
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the synergistic effect of lactic acid bacteria, enzyme preparations and soluble carbohydrates, the additive prolongs the shelf life of fresh mu

Method used

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  • Silage paper mulberry leaf feed microbial inoculum and application method thereof
  • Silage paper mulberry leaf feed microbial inoculum and application method thereof
  • Silage paper mulberry leaf feed microbial inoculum and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Using wheat bran, fresh leaves of mulberry tree and molasses as raw materials for solid-state fermentation, a composite strain consisting of Aspergillus niger FECJX203.1 with high tannase production and Lactobacillus plantarum FECJX108 with lactic acid production was inoculated. Composite strains are solid-state fermented and dried to prepare silage leaf feed fermenting strains containing two live strains and tannase.

[0022] The solid-state fermentation raw material is 50kg, the ratio of wheat bran, fresh leaves and molasses is 10:4:0.5, the water content of the system is 50%, and it is sterilized under high temperature and high pressure at 121°C for 15 minutes;

[0023] The solid-state fermentation of wheat bran leaf molasses is as follows: the inoculum amount of the compound strain is 1%; it is produced by constant temperature fermentation at 28° C. for 6 days.

[0024] The composite strain is formed by mixing the above-mentioned Aspergillus niger FECJX203.1 bacteri...

Embodiment 2

[0034] Using wheat bran, fresh leaves of mulberry tree and molasses as raw materials for solid-state fermentation, a composite strain consisting of Aspergillus niger FECJX203.1 with high tannase production and Lactobacillus plantarum FECJX108 with lactic acid production was inoculated. Composite strains are solid-state fermented and dried to prepare silage leaf feed fermenting strains containing two live strains and tannase.

[0035] The solid-state fermentation raw material is 40kg, the ratio of wheat bran, fresh leaves and molasses is 10:3:1, the water content of the system is 54%, and it is sterilized under high temperature and high pressure at 121°C for 15 minutes;

[0036] The solid-state fermentation method of wheat bran leaves and molasses is as follows: the inoculum amount of the compound strain is 2%, and it is produced by constant temperature fermentation at 30° C. for 5 days.

[0037] The composite strain is formed by mixing the above Aspergillus niger FECJX203.1 ba...

Embodiment 3

[0047] Using wheat bran, fresh leaves of mulberry tree and molasses as raw materials for solid-state fermentation, a composite strain consisting of Aspergillus niger FECJX203.1 with high tannase production and Lactobacillus plantarum FECJX108 with lactic acid production was inoculated. Composite strains are solid-state fermented and dried to prepare silage leaf feed fermenting strains containing two live strains and tannase.

[0048] The solid-state fermentation raw material is 20kg, the ratio of wheat bran, fresh mulberry leaves and molasses is 10:1:2, the water content of the system is 58%, and it is sterilized under high temperature and high pressure at 121°C for 15 minutes;

[0049] The solid-state fermentation method of wheat bran leaves and molasses is as follows: the inoculum amount of the compound strain is 3%; it is produced by constant temperature fermentation at 32° C. for 4 days.

[0050] The composite strain is formed by mixing the above-mentioned Aspergillus nige...

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Abstract

The invention discloses a silage paper mulberry leaf feed microbial inoculum and a preparation method thereof. The method takes wheat bran, fresh paper mulberry leaves and molasses as culture medium raw materials, adopts a solid-state fermentation technology, scientifically matches aspergillus niger FECJX203.1 with lactobacillus plantarum FECJX108 so as to prepare the silage paper mulberry leaf feed microbial inoculums. The microbial inoculums is used for inoculation according to a certain proportion for silage fermentation of paper mulberry leaves, can degrade tannin content and crude fiber content of paper mulberry and improve nutrients, digestibility and flavor quality of paper mulberry leaves, and simultaneously has the effects of regulating the microbial structure of intestinal tractsof animals and improving intestinal tract functions of animals.

Description

technical field [0001] The invention relates to a microbial additive, in particular to the preparation and application method of a silage bacterial agent for degrading tree leaf tannin, which belongs to the technical field of silage fermentation. technical background [0002] At present, the raw materials for pig breeding in my country are mainly food crops such as soybean meal and corn, and the phenomenon of competition with people for food is serious. Recently, due to the reduction of soybean imports, the prices of protein feed materials such as corn, soybean meal, and fish meal have risen, resulting in continuous increase in feed production costs, resulting in rising breeding costs. The problem of protein feed shortage in my country has not been alleviated. Many scholars have studied various grass and woody new protein feeds, especially the shoots and leaves of shrubs with high protein content in the southern hilly areas are good sources of woody protein raw materials. B...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A23K30/18C12R1/685C12R1/25
CPCA23K30/18C12N1/14C12N1/20A23V2400/169
Inventor 刘成梅付桂明邓丽萍罗舜菁
Owner NANCHANG UNIV
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