Application of TRPV5 gene SNP locus in detecting susceptibility to heavy-metal poisoning

A heavy metal and susceptibility technology, applied in the field of biomedical molecular detection, to achieve the effect of controlling detection costs, reducing manual operations, and taking into account accuracy

Active Publication Date: 2019-03-29
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Currently, no studies have shown that TRPV5 gene polymorphisms are associated with susceptibility to heavy metal (such as lead) poisoning

Method used

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  • Application of TRPV5 gene SNP locus in detecting susceptibility to heavy-metal poisoning
  • Application of TRPV5 gene SNP locus in detecting susceptibility to heavy-metal poisoning
  • Application of TRPV5 gene SNP locus in detecting susceptibility to heavy-metal poisoning

Examples

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Embodiment 1

[0056] Example 1. The two newly discovered SNP sites on the TRPV5 gene are related to the susceptibility to heavy metal (lead) poisoning (case-control analysis)

[0057] 1. Taking 1301 Chinese Han workers who have been exposed to lead-contaminated work environment for a long time (over 2 years) as the research object, according to GBZ 37-2002 Occupational Chronic Lead Poisoning Diagnostic Criteria, people with blood lead ≥400μg / L are regarded as high In the blood lead group (ie, lead poisoning susceptible group, case group), people with blood lead 0.05).

[0058] The above-mentioned blood lead determination uses the American ESA company 3010B blood lead analyzer by anodic stripping voltammetry to determine the blood lead content.

[0059] 2. Detect the g.103 (C>T) and g.654 (G>A) genotypes of the above-mentioned high blood lead group (lead poisoning susceptible group) and low blood lead group. It is known that the SNP site g.7240 (G>A) that is not related to lead poisoning susceptib...

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Abstract

The application discloses application of a TRPV5 gene SNP locus in detecting susceptibility to heavy-metal poisoning. The SNP locus is the 654th nucleotide of TRPV5 genes, wherein G is greater than A,the sequence of the TRPV5 genes is as shown in SEQ ID No.10. G and A of the SNP locus are significantly different between a high blood-lead group and a low blood-lead group, wherein G is a susceptible allele or high-risk allele of lead poisoning, A is a non-susceptible allele or low-risk allele of the lead poisoning, so that the base type of the locus is detected and the genotype of the locus isdetermined, so that the susceptibility to heavy-metal (such as lead) poisoning or the risks of the heavy-metal poisoning can detected, patients with the heavy-metal poisoning can be screened, susceptible genes of the heavy-metal poisoning can be identified or assisted, and single nucleotide polymorphism associated with the heavy-metal poisoning or metabolism can be detected. The application and product provided by the invention are of great value in identifying the susceptible population of the heavy-metal poisoning, thereby reasonably preventing the heavy-metal poisoning.

Description

Technical field [0001] The invention belongs to the technical field of biomedical molecular detection, and specifically relates to the application of a TRPV5 gene SNP site in detecting heavy metal poisoning susceptibility. Background technique [0002] The original meaning of heavy metals refers to metals with a specific gravity greater than 5 (generally speaking, metals with a density greater than 4.5 grams per cubic centimeter), including gold, silver, copper, iron, mercury, lead, and cadmium. Heavy metals accumulate in the human body to a certain extent. Will cause chronic poisoning. In terms of environmental pollution, heavy metals mainly refer to heavy elements with significant biological toxicity such as mercury (mercury), cadmium, lead, chromium, and metalloid arsenic. Heavy metals are very difficult to be biodegraded, but on the contrary, they can be enriched thousands of times under the biological amplification of the food chain, and finally enter the human body. Heavy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 叶秀峰刘文兰徐迹刘瑾许蕴余志英陈光福
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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