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Metabolic product extraction method and additive

A technology of metabolites and extraction methods, which is applied in the field of agricultural product processing, can solve the problems of little research on simultaneous extraction methods, and achieve the effects of improving purity and yield, high activity, and increasing extraction rate

Inactive Publication Date: 2019-04-02
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in some current extraction methods, more emphasis is placed on the high extraction rate of a single component, but there is little research on the simultaneous extraction of multiple components in Cordyceps militaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] (1) Extraction of metabolites in Cordyceps militaris: 2kg of Cordyceps militaris raw material is pulverized, crosses 40 mesh sieves, then adds deionized water to extract, and then extracts are filtered to obtain Cordyceps militaris metabolites extract; extraction condition is solid-liquid ratio For 1kg: 5L, the temperature is 50°C, the extraction time is 1, and the number of extractions is 1 time.

[0103] (2) Separation of metabolites in Cordyceps militaris:

[0104] Concentrating the extract of metabolites of Cordyceps militaris to obtain a concentrated solution of extracts of metabolites of Cordyceps militaris, adding 3 times the amount of 95% ethanol, precipitating for 24 hours, centrifuging, collecting the precipitate layer and collecting the supernatant. Redissolve the centrifuged part in deionized water, repeat the above operation once, collect the precipitate layer and supernatant, and combine the supernatant. Freeze-dry the centrifuged part to obtain the polys...

Embodiment 2

[0110] (1) Extraction of metabolites in Cordyceps militaris: 4kg of Cordyceps militaris raw material is pulverized, crosses 120 mesh sieves, then adds deionized water to extract, and then extracts are filtered to obtain Cordyceps militaris metabolites extract; extraction condition is solid-liquid ratio It is 1kg: 50L, the temperature is 85°C, the extraction time is 3h, and the extraction frequency is 3 times.

[0111] (2) Separation of metabolites in Cordyceps militaris:

[0112] Concentrate the extract of metabolites of Cordyceps militaris to obtain a concentrated solution of extracts of metabolites of Cordyceps militaris, add 3 times the amount of 95% ethanol, precipitate for 48 hours, centrifuge, collect the precipitate layer and collect the supernatant. Redissolve the centrifuged part in deionized water, repeat the above operation 3 times, collect the precipitated layer and supernatant, and combine the supernatant. Freeze-dry the centrifuged part to obtain the polysacchar...

Embodiment 3

[0118] (1) Extraction of metabolites in Cordyceps militaris: 10 kg of Cordyceps militaris raw material is pulverized, crosses 60 mesh sieves, then adds deionized water to extract, and then extracts are filtered to obtain Cordyceps militaris metabolites extract; extraction condition is solid-liquid ratio For 1kg: 20L, the temperature is 75°C, the extraction time is 2h, and the extraction frequency is 2 times.

[0119] (2) Separation of metabolites in Cordyceps militaris:

[0120] Concentrating the extract of metabolites of Cordyceps militaris to obtain a concentrated solution of extracts of metabolites of Cordyceps militaris, adding 3 times the amount of 95% ethanol, precipitating for 36 hours, centrifuging, collecting the precipitate layer and collecting the supernatant. The centrifuged part was redissolved in deionized water, and the above operation was repeated twice, the precipitate layer and supernatant were collected, and the supernatants were combined. Freeze-dry the ce...

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PUM

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Abstract

The invention relates to a metabolic product extraction method and an additive, and belongs to the field of agricultural product processing. The extraction method includes the steps: providing a material to be extracted. The material to be extracted includes a first liquid part substance having a first target product and a solid part substance having a second target product. Microporous resin is used for performing primary purification operation on the first liquid part substance to obtain a second liquid part substance with the first target product, and the second liquid part substance is subjected to secondary purification operation by a liquid chromatography way to obtain a third liquid part substance having a first target substance. The extraction method can achieve complete extractionof metabolic products from cordyceps militaris.

Description

technical field [0001] The invention relates to the field of agricultural product processing, in particular to a method for extracting metabolites and an additive. Background technique [0002] Cordyceps militaris (Cordyceps militaris L.Link) is also called militaris, Cordyceps militaris, and Cordyceps militaris. It belongs to the same species as Cordyceps sinensis, and is a medicinal and edible fungus with rich nutritional and medicinal values. Modern pharmacological studies have confirmed that Cordyceps militaris has various pharmacological activities such as antibacterial, anti-inflammatory, anti-tumor, anti-fatigue, anti-diabetes and improving immunity. Its medicinal and edible value is comparable to that of Cordyceps sinensis, so now it is generally used artificially cultivated Cordyceps militaris to replace natural Cordyceps sinensis. [0003] Cordyceps militaris contains adenosine, cordycepin, cordycepic acid, polysaccharides and other biologically active ingredient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H3/04C07H19/167C07C29/74C07C31/26C08B37/00A23L31/00A23L33/00
CPCA23L31/00A23L33/00C07C29/74C07H1/08C07H3/04C07H19/167C08B37/0003A23V2002/00
Inventor 于瑞涛陶燕铎邵赟梅丽娟江磊李世华杨小兵文怀秀刘增根
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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