Kit used for detecting TEL-AML1 fusion gene

Abstract

The present invention relates to a kit used for detecting a TEL-AML1 fusion gene. The kit comprises a TEL-AML1 fusion gene amplification detection reagent kit and the TEL-AML1 fusion gene amplification detection reagent kit comprises primer pairs used for amplifying the TEL-AML1 fusion gene and detection probes. The kit designs the primers and probes, and corresponding controls suitable for a microdroplet type digital PCR system, can be used for a detection system based on the microdroplet type digital PCR system, and is highly automated in detection processes, good in effects, very convenientin operation, easy to use and suitable for clinical applications and promotion.

Description

technical field [0001] The invention relates to the detection field of TEL-AML1 fusion, more particularly, relates to a kit for detecting TEL-AML1 fusion gene. Background technique [0002] Acute lymphoblastic leukemia (ALL) accounts for about 75% of childhood leukemia. TEL-AML1 fusion gene is a common fusion gene in childhood leukemia, and the leukemia caused by it accounts for 20%-25% of childhood acute lymphoblastic leukemia. Formed by t(12;21)(p13;q22), that is, the TEL (Trans location Ets Leukemia) helix-loop-helix region located on chromosome 12 is fused to the DNA of AML1 (Acute Myeloid Leukemia 1) located on chromosome 21 Formed by binding and activating regions, the formation of the TEL-AML1 fusion gene is often accompanied by the loss of another allele, and translocations and deletions complicate the mechanism of leukemia. It is found in 12%-28% of B-lineage ALL, but only in B precursor cell ALL (BCP-ALL), not in mature B-ALL and T-ALL. The incidence of TEL-AML1 ...

AI Technical Summary

Problems solved by technology

Although the result of fluorescence in situ hybridization (FISH) method is relatively intuitive, the operation is complex and cumbersome, involving a wide variety of reagents, time-consuming, and requires large-scale equipment such as fluorescence microscopes and diagnostic opinions from pathologists, and has strict requirements for testing institutions. Not conducive to the rapid detection of clinical samples

The results of RT-PCR cannot be monitored in real time, and the products need to be detected by gel electrophoresis, which is likely to cause false positive and false negative results, and the RT-PCR method is the final quantitative method, which cannot accurately quantify the amount of initial template

Fluorescent PCR technology has the advantages of high sensitivity of PCR, specificity of DNA hybridization and accurate quantification of spectral technology. The results are intuitive and can directly monitor the changes in the PCR process. Compared with ordinary PCR, the results can be observed in real time, and the products are not Gel electrophoresis detection is required, and completely closed-tube operation effectively reduces the risk of PCR product contamination, but qPCR can only achieve relative quantification unless a standard curve is generated with a standard, which greatly increases the cost for the initial The amount of template may not be accurately quantified, and it is difficult to detect small residues, and the missed detection rate for positive samples with a very low fusion ratio is very high

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