Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A cell penetrating peptide and bifidobacterium expressing oral interferon

A technology of bifidobacteria and penetrating peptides, which is applied in the field of biomedicine to achieve the effects of improving therapeutic effect, easy preparation, and enhancing therapeutic effect

Active Publication Date: 2022-03-08
SHANGHAI HUAXIN HIGH BIOTECH
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this phospholipid bilayer is essential for cell survival and function, it poses challenges for the exchange of cargo molecules inside and outside the cell

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A cell penetrating peptide and bifidobacterium expressing oral interferon
  • A cell penetrating peptide and bifidobacterium expressing oral interferon
  • A cell penetrating peptide and bifidobacterium expressing oral interferon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Screening HXCPP peptide sequence

[0061] Using phage in vivo display technology to screen for CPPs that can penetrate the skin. The specific method is,

[0062] 1) Applying the phage peptide library on the skin surface of nude mice through transdermal administration;

[0063] 2) after a period of transdermal, blood is drawn from the mouse body;

[0064] 3) Add Escherichia coli to the blood, let the phage infect the Escherichia coli, and then culture it;

[0065] 4) Finally count the plaques and calculate the phage titer.

[0066] The first round of screening is carried out according to the above steps. Usually, many non-specific phages or phages with weak transdermal ability will be screened in the first round of screening, and the number is large. Therefore, repeated screening is required. By repeating the above method. After 3-4 rounds of screening, the phages were enriched to a certain purity.

[0067] Then, the polypeptide sequence is obtained by extracting ...

Embodiment 2

[0072] (1) Construction of recombinant vector

[0073] The genes encoding HXCPP and IFNα-2b were constructed on the shuttle vector pHX101 (with Escherichia coli and Bifidobacterium replicons at the same time), transformed into competent cells BL21 (DE3), and obtained recombinant plasmid pHX101-HXCPP after enzyme digestion, sequencing, and screening -IFNα-2b, meanwhile, only the IFNα-2b gene fragment was constructed on pHX101-IFNα-2b, as a control.

[0074] Wherein, the amino acid sequence of HXCPP is shown in SEQ ID NO:1; the amino acid sequence of HXCPP-IFNα-2b fusion protein is shown in SEQ ID NO:2.

[0075] (2) Construction of recombinant engineering bacteria

[0076] The above plasmids were respectively transformed into Bifidobacterium longum, cultured anaerobically at 37°C in sugar-free BM solid medium, picked colonies for PCR identification, and sequenced the positive strains for further identification.

[0077] Identify the recombinant engineering bacteria with correc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biomedicine, and discloses a cell-penetrating peptide and a bifidobacterium capable of expressing oral interferon. The cell-penetrating peptide provided by the invention is a peptide that mediates the delivery of biologically active molecules into cells. The cell-penetrating peptide The amino acid sequence of the perpeptide includes the sequence shown in SEQ ID NO:1. The fusion protein of the above-mentioned cell penetrating peptide and interferon can be expressed in bifidobacteria capable of expressing oral interferon, and the cell penetrating peptide is linked to the N-terminal of the interferon. The cell-penetrating peptide provided by the present invention has higher transdermal activity, and the prepared Bifidobacterium longum that can express oral interferon can be colonized in the intestinal tract of animals, and after oral administration of an inducer, the expression can penetrate the intestinal epithelium and enter The interferon α‑2b in the body has good skin penetration and can enhance the therapeutic effect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a cell-penetrating peptide and a bifidobacterium capable of expressing oral interferon. Background technique [0002] Transdermal drug delivery refers to a drug delivery system that penetrates the stratum corneum of the skin and enters the capillaries to enter the blood circulation after the drug is administered on the skin surface, and has a systemic therapeutic effect. As a special route of administration, it has unique advantages compared with traditional oral administration and injection. Good drug efficacy, and reduce individual differences in medication; maintain a constant and effective blood drug concentration, avoid the peak and valley phenomenon of blood drug concentration, and reduce toxic and side effects; prolong the drug action time, reduce the number of administrations, and make it easy for most patients to accept; no pain , so that the convenience, patients...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12N15/11C07K19/00C12N1/21C12N15/74A61K47/42A61K47/64
CPCA61K47/42A61K47/64C12N15/74C07K7/08C07K14/555C07K14/56C07K14/565C07K14/57C07K2319/10
Inventor 李冠英张德宝李海红刘惠
Owner SHANGHAI HUAXIN HIGH BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com