Differential raman spectrometer capable of deducting fluorescence in real time and method thereof
A Raman spectrometer and Raman spectroscopy technology, applied in the field of spectroscopy, can solve the problems such as the inability to effectively deduct the fluorescence background, the large difference in the fluorescence background, the uneven composition of the measured object, etc., to achieve the function of real-time deduction of fluorescence and improve sample detection. Effect, the effect of enhancing the local signal of Raman characteristic peaks
Pending Publication Date: 2019-04-05
浙江澍源智能技术有限公司
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The composition of the measured object is uneven, the measured surface is uneven, or even movable, and the spectral acquisition time is several seconds, tens of seconds or even longer. During this process, the sample is easily displaced slowly due to factors such as mechanical vibration and thermal deformation, resulting in The change of the measured position will affect the process of fluorescence emission and fluorescence collection, and the fluorescence background of the two measurements will be greatly different, so that the fluorescence background cannot be effectively deducted
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[0045] The sample to be tested is gypsum crystal. The gypsum crystal is placed near the focus of the focal plane of the short-focus lens 8. The surface of the gypsum crystal will spontaneously form a layer of fluorescent layer in the air. The fluorescent signal spreads throughout the Raman spectrum, forming background noise and interfering with Raman. spectrum. When the gypsum crystal is excited by 785nm or 784nm laser, the wave number is 200~1300cm -1 There are 6 Raman characteristic peaks in the range.
[0046] Set the integration time as t=100ms, N=5, λ 1 =785nm, λ 2=784nm, according to the steps described above, 5 groups of S (785nm) and 5 groups of S (784nm) were respectively obtained, and the spectrum was smoothed by the Savitzky-Golay convolution algorithm. The window of the Savitzky-Golay convolution algorithm is 5, and the order is 9. Perform Lagrange interpolation on the Raman characteristic peaks of the smoothed data to obtain fcn i (λ 1 ) and fcn i (λ 2 ), r...
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The invention discloses a differential raman spectrometer capable of deducting fluorescence in real time and a method thereof, and belongs to the technical field of optical measurement. The differential raman spectrometer comprises a control unit, a dual-wavelength laser device, a dual-channel optical switcher, a detector, and the like. A dual-wavelength laser emits dual-wavelength lasers to successively stimulate a sample to generate a scattered spectrum so as to obtain raman data, then reconstruction treatment is conducted on the dual-wavelength raman data through the control unit, the reconstruction treatment comprises datum smoothness treatment, characteristic peak local interpolation treatment and raman reconstruction treatment, and thus the raman spectrometer with the fluorescence being deducted is obtained. The differential raman spectrometer capable of deducting the fluorescence in real time and the method thereof have the advantages that the fluorescence background in the raman spectrometer measurement process is deducted in real time, and the sample detection effect is improved.
Description
technical field [0001] The invention relates to a differential Raman spectrometer capable of subtracting fluorescence in real time and a method thereof, belonging to the field of spectrum technology. Background technique [0002] Raman spectroscopy detection has outstanding advantages such as fast detection speed, no damage to samples, and simple sample preparation. It is a widely used detection method. And with the maturity of Raman spectroscopy detection technology, its miniaturization, portability and intelligence have gained more favor and development. Portable Raman spectrometers have the advantages of small size, low cost, and on-site real-time detection, but they inevitably encounter some obstacles, among which fluorescence interference is the biggest obstacle. [0003] The physical mechanism of Raman spectroscopy is Raman scattering, but the probability of Raman scattering is very small, and the intensity of Raman light is much smaller than that of fluorescence. Ev...
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IPC IPC(8): G01N21/65G01N21/01G01J3/44G01J3/02
CPCG01J3/0262G01J3/44G01N21/01G01N21/65G01J2003/4424
Inventor 聂荣志何智亮彭波储涛熊泉赵子健张广维
Owner 浙江澍源智能技术有限公司