A crystallization auxiliary film for maldi target plate

Abstract

The invention relates to an MALDI target plate crystallization auxiliary film. The crystallization auxiliary film comprises a side wall hole array corresponding to the target plate array, a colloid layer for preventing liquid from leaking on the surface of the target plate and an anti-pollution release film. According to the invention, the crystallization auxiliary film is pasted on the surface ofthe MALDI target plate, and the side wall hole of the auxiliary film and the surface of the target point form a cylindrical micro-container. A conventional sample and a matrix are loaded into a micro-container, and under the action of surface tension of side walls on liquid, co-crystallization formed by the conventional sample and the matrix is better in the central position of a target point. Inthe process of MALDI batch point target analysis, the dissociation effect of laser analysis is remarkably improved, and meanwhile the sensitivity and repeatability of MALDI analysis can be improved to a certain extent.

Description

technical field [0001] The invention relates to the field of analysis and detection instruments, in particular to a crystallization auxiliary film of a MALDI target plate. Background technique [0002] Matrix-assisted laser desorption ionization (MALDI) is a soft ionization technology developed from laser desorption (LD). The basic principle is that a matrix is ​​applied on the surface of the sample to be tested, and after the co-crystallization of the two is completed, the matrix effectively absorbs laser energy of a certain wavelength and transmits it evenly to the sample to be tested, so that it can complete the instantaneous gas flow. ionization and ionization for subsequent mass spectrometry analysis. [0003] With its unique ionization method, MALDI can easily ionize macromolecules, such as proteins, peptides, DNA, polysaccharides, glycoproteins, polymers, etc. At the same time, MALDI does not have high requirements for samples and can tolerate the presence of higher...

AI Technical Summary

Problems solved by technology

In the crystallization process, the shape of the hemisphere determines that the crystallization effect is better at the edge position than at the center position, resulting in unsatisfactory dissociation effects when the laser irradiates the center of the target point during the scanning process, thus affecting the quality of the spectrum. lead to poor repeatability of the experiment

In addition, during repeated cleaning and use of the metal target plate, it is easy to cause scratches on the surface, which will affect the formation of subsequent crystals.

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