Rapid and efficient breeding method for peanut variety with high oleic acid content

Abstract

The invention relates to the technical field of peanut breeding and provides a rapid and efficient breeding method for a peanut variety with high oleic acid content. According to the method, the oleicacid content of single-grain peanuts is detected by near infrared on the basis of the law of the number of AhFAD2 gene mutations in single-grain peanut seeds and the oleic acid content, the oleic acid content can be selected in the early stage of hybridization by setting different selection conditions of the oleic acid content without dependence on molecular markers, and the peanut variety with the high oleic acid content is rapidly and efficiently bred; the method is rapid, efficient, simple and easy to operate, the breeding progress of the peanut variety with the high oleic acid content iseffectively accelerated, breeding efficiency is improved, and cost is reduced to a great degree.

Description

technical field [0001] The invention belongs to the technical field of peanut breeding, and in particular relates to a method for quickly and efficiently cultivating high-oleic acid peanut varieties. Background technique [0002] Peanut (Arachis hypogaea L.) is one of the main oilseed and economic crops in the world. Among the domestic bulk oil crops, the total peanut production, oil production per unit area, and planting industry output rank first. When only half of the total peanut production is used for oil extraction, the annual peanut oil output is about 2.7 million tons, accounting for 100% of domestic vegetable oil production. A quarter of the production, it is the second largest source of domestic vegetable oil (after rapeseed oil). [0003] Fatty acid composition is a key quality trait that affects the nutritional and health value, processing characteristics and efficiency, storage resistance and market competitiveness of peanuts and their processed products. Olei...

AI Technical Summary

Problems solved by technology

However, the above method requires PCR, enzyme digestion and / or relies on expensive fluorescent quantitative equipment and probes, which is time-consuming, laborious and cumbersome to operate, and requires professionals, expensive equipment and reagents, and the detection cost is high; meanwhile, due to the The high similarity of the gene sequence will lead to a certain proportion of false positives in the identification results, which limits the application of the above method in peanut high oleic acid breeding

Images