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Bacillus subtilis GUMT332 and application thereof

A technology for Bacillus subtilis and tobacco black shank, which can be used in application, bacteria, fungicides, etc., and can solve problems such as increased drug dosage, pollution, and loss in the field

Active Publication Date: 2019-04-09
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, capsicum anthracnose is one of the main diseases in capsicum production, which can cause damage throughout the growth period of capsicum, causing great losses
Guizhou Province is one of the main cultivation areas of pepper in my country. The long-term continuous cropping of pepper has caused the continuous expansion of the incidence of anthracnose, seriously affecting the income of farmers
At present, chemical pesticides are mainly used to prevent and control diseases in production. The resistance of pathogenic bacteria is increasing year by year, and the amount of pesticides used in the field is also increasing accordingly, which can easily cause pesticide residues and environmental pollution.

Method used

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  • Bacillus subtilis GUMT332 and application thereof
  • Bacillus subtilis GUMT332 and application thereof
  • Bacillus subtilis GUMT332 and application thereof

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Experimental program
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Embodiment Construction

[0016] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:

[0017] 1. Isolation and screening of Bacillus subtilis GUMT332

[0018] 1. Materials and methods

[0019] 1.1 Materials

[0020] From 2017 to 2018, the plots with severe tobacco black shank disease were selected from Meitan County, Zunyi City, Guizhou Province, and the rhizosphere soil of healthy tobacco plants was collected. The 5-point sampling method was used to mix soil samples from the same plot to count as 1 soil sample. Soil samples were collected in sterile ziplock bags, and soil collection tools were sterilized with 75% alcohol to avoid soil microbial mixing between different fields.

[0021] 1.2 Medium

[0022] LB medium is used for the isolation and preservation of Bacillus; PDA medium is used for plate confrontation culture experiments; protease detection medium, cellulase detection medium, siderophilic detection medium, phosphatase detection medi...

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Abstract

The invention discloses bacillus subtilis GUMT332 collected in CCTCC (China Center for Type Culture Collection), with the collection number of CCTCC NO:M 2018874. The invention also discloses an application of the bacillus subtilis GUMT332 in control of phytophthora nicotianae. The bacillus subtilis GUMT332 has obvious capacity for antagonizing the phytophthora nicotianae and chili anthracnose.

Description

technical field [0001] The invention relates to a bacillus subtilis GUMT332 and an application thereof, belonging to the technical field of biological control. Background technique [0002] Tobacco black shank (Phytophthora nicotianae) is one of the most destructive soil-borne diseases in tobacco production in Guizhou. The economic loss caused by this disease is nearly ten million yuan every year, seriously affecting the economic income of tobacco farmers. At present, chemical control is mostly used in production, but the main chemical has a long service life, the resistance of pathogenic bacteria is increasing year by year, and the negative effects of pesticide residues and environmental pollution are also gradually increasing. Therefore, the use of biological control to control tobacco diseases is a hot research field at present. [0003] Bacillus (Bacillus spp.) is currently the most commonly used microbial species in biological control research. Bacillus has the prospec...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/125
CPCA01N63/00C12R2001/125C12N1/205
Inventor 彭丽娟丁海霞余水
Owner GUIZHOU UNIV
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