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A cell culture medium suspension oil preparation of artemether and its application in cell culture medium

A technique for artemether and culture medium, which is applied to a cell culture medium suspending oil agent and its application field in cell culture medium, can solve the problems that the solubility of artemether cannot meet the experimental requirements, etc., so as to avoid composition change or loss. , the effect of fast dissolution

Active Publication Date: 2022-04-26
HAINAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the experiment, we chose absolute ethanol and olive oil as solvents, but we also found that ethanol or olive oil artemether not only precipitated in the cell culture medium, but also the solubility of artemether in the cell culture medium could not reach the experimental level. need

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of a cell culture medium suspension oil preparation of artemether, comprising the steps of:

[0035] a) Mix absolute ethanol and olive oil according to the mixing ratio of absolute ethanol and olive oil is 1:3, let stand for 45 minutes, the upper part is the absolute ethanol layer, and the lower part is the olive oil layer;

[0036] b) Take the olive oil layer liquid, according to the mass volume ratio of artemether powder and olive oil layer liquid is 333mg / ml, add it into the weighed artemether powder, dissolve the artemether, the dissolution time is 7 minutes, and become artemether Ether solution, the solubility of artemether solution is 333mg / ml;

[0037] c) Take 60ul artemether solution and add it to 1ml cell culture medium, no artemether is precipitated, shake it at room temperature for 5 minutes with an oscillator, and shake it into a cell culture medium suspension oil of artemether; then in cell culture , using a self-made shaking oscillato...

Embodiment 2

[0040] A preparation method of a cell culture medium suspension oil preparation of artemether, comprising the steps of:

[0041] a) Mix absolute ethanol and olive oil according to the mixing ratio of absolute ethanol and olive oil is 1:1, let stand for 15 minutes, the upper part is the absolute ethanol layer, and the lower part is the olive oil layer;

[0042] b) Take the olive oil layer liquid, according to the mass volume ratio of artemether powder and olive oil layer liquid is 340mg / ml, add it into the weighed artemether powder, dissolve the artemether, the dissolution time is 5 minutes, and become artemether Ether solution, the solubility of artemether solution is 340mg / ml;

[0043] c) Take 60ul artemether solution and add it to 1ml cell culture medium, no artemether is precipitated, shake it at room temperature for 5 minutes with an oscillator, and shake it into a cell culture medium suspension oil of artemether; then in cell culture , using a self-made shaking oscillato...

Embodiment 3

[0046] A preparation method of a cell culture medium suspension oil preparation of artemether, comprising the steps of:

[0047] a) Mix absolute ethanol and olive oil according to the mixing ratio of absolute ethanol and olive oil is 1:6, let stand for 1 hour, the upper part is the absolute ethanol layer, and the lower part is the olive oil layer;

[0048] b) Take the olive oil layer liquid, according to the mass volume ratio of the artemether powder and the olive oil layer liquid is 320mg / ml, add the weighed artemether powder, dissolve the artemether, the dissolution time is 9 minutes, and become artemether Ether solution, the solubility of artemether solution is 320mg / ml;

[0049] c) Take 60ul artemether solution and add it to 1ml cell culture medium, no artemether is precipitated, shake it at room temperature for 5 minutes with an oscillator, and shake it into a cell culture medium suspension oil of artemether; then in cell culture , using a self-made shaking oscillator to...

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Abstract

The invention discloses a preparation method of artemether cell culture medium suspension oil and its application in cell culture medium, comprising the following steps: a) mixing absolute ethanol and olive oil according to the ratio, and standing for 5 Minutes to 1 hour, the upper part is anhydrous ethanol layer, and the lower part is an olive oil layer; b) Take the olive oil layer liquid and add it to the weighed artemether powder to dissolve the artemether, and the dissolution time is less than 10 minutes to become artemether Dissolving solution; c) Add artemether dissolving solution into cell culture medium, artemether is not precipitated, and shake to form artemether cell culture medium suspension oil. The artemether cell culture medium suspension oil prepared by the invention is safe and non-toxic, and the artemether dissolves quickly without precipitation. Moreover, the artemether in the suspension oil is in the state of suspension oil, which can promote the contact with the cells in the cell culture medium, thereby ensuring that the drug concentration of artemether in the cell culture medium is as high as 20 mg / ml, which is beneficial to the production of artemether. Study on the physical action of methyl ether itself.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a cell culture medium suspension oil preparation of artemether and its application in a cell culture medium. Background technique [0002] Artemether powder cannot be directly dissolved in cell culture medium. Artemether injections are difficult to buy in the market. Therefore, in cell experiments, it becomes a difficult problem to dissolve artemether powder in the cell culture medium. In many literatures, DMSO (dimethyl sulfoxide) is commonly used as a solvent for artemether in cell experiments. In the experiment, we found that the artemether dissolved in DMSO was not only precipitated when added to the cell culture medium, but also the solubility of artemether in the cell culture medium could not meet the experimental requirements. According to the literature, absolute ethanol and olive oil are used as solvents, and the solubility of artemether is relatively high. In th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12Q1/02
CPCG01N33/5008C12N5/0018G01N2500/10C12N2501/999C12N2503/02
Inventor 范志刚陈妮刘嫱符怀艺王琳符瑞祥谢杨巫翠萍
Owner HAINAN MEDICAL UNIV
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