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Composition containing miR-124-3P and application thereof in drug for inducing neuron formation

A composition and drug technology, which is applied in the direction of drug combination, nervous system diseases, medical preparations containing active ingredients, etc., can solve the problems of unfavorable central nervous system regeneration and repair, uneconomical convenience, high cost, etc., and achieve beneficial regeneration and repair, The effect of inducing few components and simple steps

Active Publication Date: 2019-04-26
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the best treatment time after CNS damage is in the subacute stage of injury. The existing methods take a long time to differentiate into neurons, and the steps are cumbersome. Conducive to the regeneration and repair of the central nervous system
Secondly, there are many induction components required in the induction process, and there are many types of small molecule compounds. It is necessary to add 6 kinds of small molecules, and the cost is high.
In short, it is not economical and convenient

Method used

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  • Composition containing miR-124-3P and application thereof in drug for inducing neuron formation
  • Composition containing miR-124-3P and application thereof in drug for inducing neuron formation
  • Composition containing miR-124-3P and application thereof in drug for inducing neuron formation

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Embodiment 1

[0045] Experimental material: Rat As, sourced from self-made or purchased As primary cells.

[0046] The preparation method includes antibody method and trypsin method. The method adopted in the present embodiment is as follows: Wistar large suckling rats (born 1-3 days, 10) were placed in 75% ethanol and soaked and disinfected for 15 minutes. After cleaning with PBS, the brain was stripped with micro scissors and micro tweezers. Remove the hippocampus, meninges, etc., and peel off the cerebral cortex in a non-adherent cell dish containing 15ml of PBS. Break up the tissue and digest with papain 40μl Papain (40.4mgP / ml, Worthington, LS003127) + 100μl DNAse I (4mg / ml, Worthington, LS002006) for 45min at 34°C with a shaker, 6ml FBS (TransSerum EQ Fetal BovineSerum, FS201-02) and 8ml 10X EBSS (Sigma, E7510) to stop the digestion, 1500rpm, 5min centrifugation to collect the digested and separated cells (about 2ml); use DME / F12 with a volume fraction of 5% FBS to suspend the cells ...

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Abstract

The invention provides a composition containing miR-124-3P and application thereof in a drug for inducing neuron formation, and belongs to the technical field of central nerve injury repairing. The composition comprises the miR-124-3P and an inducer, wherein the inducer is selected from one or several of a JAK (Janus Kinase) inhibitor, a p38-MAPK (Mitogen-activated Protein Kinase) inhibitor and anadenylate cyclase activator. The time for trans-differentiating reactive astrocyte into neurons by utilizing the composition provided by the invention is short, the steps are simple and convenient, induction components are less, and the composition is economic, effective and convenient.

Description

technical field [0001] The invention belongs to the technical field of central nervous system injury repair, and in particular relates to a composition including miR-124-3P and its application in inducing neuron formation and preparing drugs for reducing glial scar formation. Background technique [0002] Astrocyte (As) is the main component of glial cells in the vertebrate central nervous system (Central nervous system, CNS). Under normal physiological conditions, As plays an extremely important role in the structural support of the CNS, the nutrient metabolism of neurons, and the regulation of cerebral blood flow. However, in CNS disease or injury, in addition to causing axonal rupture and functional neuron necrosis at the lesion site, a series of phenotypic changes will occur in the As around the lesion, and the naive As in the resting state is destroyed. Injury stimulation activates, swelling and hypertrophy of the cell body occurs, and becomes reactive astrocytes (RAs)...

Claims

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Application Information

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IPC IPC(8): A61K31/7088A61K31/519A61K31/4439A61K31/352A61P25/00
CPCA61P25/00A61K31/352A61K31/4439A61K31/519A61K31/7088
Inventor 池光范李玉林郑洋洋许侃候宜徐金影
Owner JILIN UNIV
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