Preservation solution and preparation process and application method thereof
A preparation process and technology of preservation solution, which is applied in the field of preservation solution and cell preservation solution, can solve the problems of inaccurate detection effect, difficulty in removing protein, difficulty in removing cell mucus, fresh blood, etc., achieve good detection effect and easy preservation conditions , Easy to detect the effect of operation
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Embodiment 1
[0059] Embodiment 1: A preservation solution, the components included and their corresponding qualities are shown in Table 1, and are prepared through the following steps:
[0060] Step 1, weigh 900g of deionized water, mix sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate heptahydrate, disodium EDTA, sodium chloride, dithiothreitol, NH 4 Cl is added to deionized water, fully mixed, then the remaining parts by weight of deionized water are added, and fully mixed again to obtain the initial mixture;
[0061] Step 2, adding a pH regulator to the initial mixture obtained in step 1, mixing fully, adjusting the pH value to the value set in Table 1, to obtain the initial product;
[0062] In step 3, the primary product obtained in step 2 is filtered through a 0.22 μm filter membrane to obtain a preservation solution.
[0063] In the above preparation process, step 1, step 2 and step 3 are operated at a humidity of 65RH% and a temperature of 28°C.
[0064] The pres...
Embodiment 6
[0067] Embodiment 6: a kind of preservation liquid, the difference with embodiment 1 is, in step one, take 800g deionized water, and dihydrogen sodium phosphate, heptahydrate disodium hydrogen phosphate, EDTA disodium, sodium chloride , dithiothreitol, NH 4 Cl was mixed well, and then 200 g of deionized water was added for mixing.
Embodiment 7
[0068] Embodiment 7: a kind of preservation solution, the difference with embodiment 1 is that in step one, take 850g deionized water, and sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate heptahydrate, disodium EDTA, sodium chloride , dithiothreitol, NH 4 Cl was mixed well, and then 200 g of deionized water was added for mixing.
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