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Preservation solution and preparation process and application method thereof

A preparation process and technology of preservation solution, which is applied in the field of preservation solution and cell preservation solution, can solve the problems of inaccurate detection effect, difficulty in removing protein, difficulty in removing cell mucus, fresh blood, etc., achieve good detection effect and easy preservation conditions , Easy to detect the effect of operation

Inactive Publication Date: 2019-04-26
HANGZHOU HEALTHSKY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the preservation process, although the traditional preservation solution can maintain a relatively stable pH value, the cell mucus, fresh blood, etc. mixed with the sample cells contain a relatively high content of protein that is difficult to remove, and even effectively removes some of the sample cells. cells encased in
During the preservation process, the traditional preservation solution is difficult to remove the cell mucus and fresh blood only through the stable pH value, so that it is difficult to fully release the effective cells covered by the cell mucus and fresh blood. In the follow-up detection, the number of effective cells is less than the actual number of effective cells, which eventually leads to inaccurate detection results

Method used

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  • Preservation solution and preparation process and application method thereof
  • Preservation solution and preparation process and application method thereof
  • Preservation solution and preparation process and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: A preservation solution, the components included and their corresponding qualities are shown in Table 1, and are prepared through the following steps:

[0060] Step 1, weigh 900g of deionized water, mix sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate heptahydrate, disodium EDTA, sodium chloride, dithiothreitol, NH 4 Cl is added to deionized water, fully mixed, then the remaining parts by weight of deionized water are added, and fully mixed again to obtain the initial mixture;

[0061] Step 2, adding a pH regulator to the initial mixture obtained in step 1, mixing fully, adjusting the pH value to the value set in Table 1, to obtain the initial product;

[0062] In step 3, the primary product obtained in step 2 is filtered through a 0.22 μm filter membrane to obtain a preservation solution.

[0063] In the above preparation process, step 1, step 2 and step 3 are operated at a humidity of 65RH% and a temperature of 28°C.

[0064] The pres...

Embodiment 6

[0067] Embodiment 6: a kind of preservation liquid, the difference with embodiment 1 is, in step one, take 800g deionized water, and dihydrogen sodium phosphate, heptahydrate disodium hydrogen phosphate, EDTA disodium, sodium chloride , dithiothreitol, NH 4 Cl was mixed well, and then 200 g of deionized water was added for mixing.

Embodiment 7

[0068] Embodiment 7: a kind of preservation solution, the difference with embodiment 1 is that in step one, take 850g deionized water, and sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate heptahydrate, disodium EDTA, sodium chloride , dithiothreitol, NH 4 Cl was mixed well, and then 200 g of deionized water was added for mixing.

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Abstract

The invention discloses a preservation solution and a preparation process and application method thereof. The preservation solution is prepared from, by weight, 1.45-1.6 parts of sodium dihydrogen phosphate dihydrate, 2.58-2.7 parts of disodium hydrogen phosphate heptahydrate, 2.36-2.5 parts of EDTA disodium, 8.1-9 parts of sodium chloride, 3-4 parts of dithiothreotol, 8.1-9 parts of NH4Cl, 1,000mL of deionized water and a pH regulator, and the pH value of the preservation solution is 6.8-7.2. According to the preservation solution, cell mucus and fresh blood carried in cell samples in the sampling process can be effectively moved, and then effective cells are fully released; in the preservation process, the cell mucus and fresh blood are not likely to affect the effective cells, and thefinally obtained detection results can achieve the accurate effect.

Description

technical field [0001] The invention relates to the technical field of cell preservation solution, more specifically, it relates to a preservation solution and its preparation process and application method. Background technique [0002] Prevention of cervical cancer has become a global public issue. When detecting cervical cancer, the cells must be sampled first. At this time, some fresh blood and cell mucus will inevitably be brought out of the taken out cell samples, or some fresh blood cells may be mixed in the taken out cell samples. During the preservation process, although the traditional preservation solution can maintain a relatively stable pH value, the cell mucus, fresh blood, etc. mixed with the sample cells contain a relatively high content of protein that is difficult to remove, and even effectively removes some of the sample cells. cells encased inside. During the preservation process, the traditional preservation solution is difficult to remove the cell muc...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/536A01N1/02
CPCA01N1/0226G01N33/536G01N33/57411
Inventor 季科研毛立新
Owner HANGZHOU HEALTHSKY BIOTECH CO LTD
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