Method for building atherosclerosis animal model of disharmony between nutritive qi and defensive qi

An atherosclerosis and animal model technology, applied in the establishment of animal models and the medical field, can solve problems such as microorganisms that cannot be excluded, and achieve the effects of saving experimental costs, shortening molding time, and shortening experimental time.

Active Publication Date: 2019-05-14
青岛市中医医院
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Problems solved by technology

It does not contain microorganisms that are pathogenic to humans or animals, but micro...

Method used

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Embodiment Construction

[0034]According to the specific experimental steps and data, this technical solution is further elaborated.

[0035] 1. Prepare animals, feed, experimental equipment and reagents.

[0036] SPF grade male Sprague-Dawley (SD) rats, body weight (220±20) g, provided by Beijing Huafukang Biotechnology Co., Ltd., rats were kept in separate cages, room temperature was (22±2)°C, relative humidity (55 ±5)%, well-ventilated, artificially controlled indoor lighting, 12h light (8:00-20:00) and dark (20:00-8:00 next day) alternate cycle, raised according to SPF level animals, fed according to requirements Ordinary rats maintained feed and high-fat feed, drank water freely, and kept the litter in the cage clean and dry.

[0037] Ordinary feed: corn 32% + peanut cake 15% + soybean meal 10% + wheat flour 20% + wheat bran 10% + fish meal 5% + rice bran 5% + salt and multivitamins 3%

[0038] High-fat feed: 3% cholesterol, 0.5% cholic acid, 10% lard, 0.2% propylthiouracil, 5% sugar, 81.3% bas...

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Abstract

The method belongs to the technical field of medical and zoological model building, and discloses a method for building an atherosclerosis animal model of disharmony between nutritive qi and defensiveqi. The method includes the following steps that an animal is anesthetized; superior thyroid ganglia is separated and exposed; a jugular sympathetic trunk is divided and ligated; suturing and disinfection are carried out; infection is prevented; the animal is fed with high-fat feed, vitamin D3 is injected into muscles, and air is blown. According to the technical scheme, the model of disharmony between nutritive qi and defensive qi is simulated through TCST, high-fat feed feeding is also used, and the atherosclerosis animal model of disharmony between nutritive qi and defensive qi is built, so that the problem that a carotid atherosclerosis animal experiment model with the symptom of disharmony between nutritive qi and defensive qi lacks in the prior art is solved. Due to the mode combining TCST, continuous air blowing, the high-fat feed and vitamin D, the model forming time of atherosclerosis can be greatly shortened, the experiment cost is reduced, the experiment time is shortened,and the method is economical, rapid to use and practical.

Description

technical field [0001] The invention belongs to the technical field of establishing medical and zoological models, and in particular relates to a method for establishing animal models of atherosclerosis caused by nutrition and health disharmony. Background technique [0002] "Suwen Qi Tongtian Theory": "The yin person stores the essence and rises urgently; the yang person protects the outside and solidifies it", which is a high-level summary of the functions of nourishing yin and defending yang. The influence of Wei Qi on the blood flow in vessels is consistent with modern medicine’s understanding that microvessels wrap around the perineural plexus and paranervous plexus to regulate vasomotor and local blood flow. It can cause contraction and relaxation of vascular smooth muscle, can change the diastolic state of blood vessels, and affect the flow of blood. The nutrient qi is injected into the veins, becomes a component of the blood and travels with the blood, exerts the fu...

Claims

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Application Information

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IPC IPC(8): A61D1/00G09B23/30
Inventor 臧运华
Owner 青岛市中医医院
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