Method for Establishing Animal Model of Atherosclerosis with Disharmony between Camp and Health

A technology of atherosclerosis and animal models, which is applied in the fields of medicine and animal model establishment, and can solve problems such as microorganisms that cannot be ruled out

Active Publication Date: 2021-08-03
青岛市中医医院
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  • Abstract
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Problems solved by technology

It does not contain microorganisms that are pathogenic to humans or animals, but microorganisms that can be vertically transmitted through the placental barrier cannot be excluded

Method used

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Embodiment Construction

[0034]According to the specific experimental steps and data, this technical solution is further elaborated.

[0035] 1. Prepare animals, feed, experimental equipment and reagents.

[0036] SPF grade male Sprague-Dawley (SD) rats, body weight (220±20) g, provided by Beijing Huafukang Biotechnology Co., Ltd., rats were kept in separate cages, room temperature was (22±2)°C, relative humidity (55 ±5)%, well-ventilated, artificially controlled indoor lighting, 12h light (8:00-20:00) and dark (20:00-8:00 next day) alternate cycle, raised according to SPF level animals, fed according to requirements Ordinary rats maintained feed and high-fat feed, drank water freely, and kept the litter in the cage clean and dry.

[0037] Ordinary feed: corn 32% + peanut cake 15% + soybean meal 10% + wheat flour 20% + wheat bran 10% + fish meal 5% + rice bran 5% + salt and multivitamins 3%

[0038] High-fat feed: 3% cholesterol, 0.5% cholic acid, 10% lard, 0.2% propylthiouracil, 5% sugar, 81.3% bas...

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PUM

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Abstract

The invention belongs to the technical fields of medicine and zoology model establishment, and discloses a method for establishing an animal model of atherosclerosis with disharmony between camp and health, comprising the following steps: anesthetize the animal; separate and expose the superior cervical ganglion; disconnect and ligate the cervical sympathetic ; suture disinfection; infection prevention; feeding high-fat feed, intramuscular injection of vitamin D3, blowing. This technical solution uses TCST to simulate the model of carotid atherosclerosis caused by nutrient-wei disharmony and high-fat feed to establish animal models of carotid atherosclerosis. Question, the method of TCST plus continuous air blowing combined with high-fat feed and vitamin D can greatly shorten the modeling time of atherosclerosis, save the cost of the experiment, shorten the time of the experiment, and is economical, fast and practical.

Description

technical field [0001] The invention belongs to the technical field of establishing medical and zoological models, and in particular relates to a method for establishing animal models of atherosclerosis caused by nutrition and health disharmony. Background technique [0002] "Suwen Qi Tongtian Theory": "The yin person stores the essence and rises urgently; the yang person protects the outside and solidifies it", which is a high-level summary of the functions of nourishing yin and defending yang. The influence of Wei Qi on the blood flow in vessels is consistent with modern medicine’s understanding that microvessels wrap around the perineural plexus and paranervous plexus to regulate vasomotor and local blood flow. It can cause contraction and relaxation of vascular smooth muscle, can change the diastolic state of blood vessels, and affect the flow of blood. The nutrient qi is injected into the veins, becomes a component of the blood and travels with the blood, exerts the fu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D1/00G09B23/30
Inventor 臧运华
Owner 青岛市中医医院
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