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A method and application of improving sensitivity of monocot crops to gibberellin inhibitors

A gibberellin and inhibitor technology is applied in the field of improving the sensitivity of monocotyledonous crops to gibberellin inhibitors, and can solve the problems of insignificant regulation effect of corn and the like

Active Publication Date: 2020-11-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Shijiean has no significant regulatory effect on maize, and when 1000mg / L DPC is used to treat maize Zhengdan 958, there is no significant difference in plant height and stem diameter from the control (Chen Yin, 2012)

Method used

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  • A method and application of improving sensitivity of monocot crops to gibberellin inhibitors
  • A method and application of improving sensitivity of monocot crops to gibberellin inhibitors
  • A method and application of improving sensitivity of monocot crops to gibberellin inhibitors

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Effect test

Embodiment 1、K409

[0053] Embodiment 1, the acquisition of K409Y ZmCPS1

[0054] The K409Y ZmCPS1 protein is a protein mutant obtained by mutating the lysine at position 409 of the amino acid sequence (Sequence 2) of the ZmCPS1 protein to tyrosine.

[0055] The K409Y ZmCPS1 gene is a nucleic acid mutant obtained by mutating the AAG base at position 1225-1227 of the ZmCPS1 gene nucleotide sequence (sequence 1) into TAT.

Embodiment 2、K409

[0056] Example 2, Application of K409Y ZmCPS1 in Improving Corn Sensitivity to Gibberellin Inhibitors

[0057] 1. Obtaining the recombinant vector

[0058] The recombinant vector pSuper1300-ZmCPS1 is the vector obtained by replacing the DNA fragment between Spe I and Kpn I of the pSuper1300 vector with the ZmCPS1 gene, and the expression of the fusion protein formed by the ZmCPS1 protein and the GFP on the carrier;

[0059] The recombinant vector pSuper1300-K409Y ZmCPS1 is a vector obtained by replacing the DNA segment between Spe I and Kpn I of the pSuper1300 vector with the K409Y ZmCPS1 gene. The fusion protein formed by K409Y ZmCPS1 protein and GFP on the carrier is expressed.

[0060] 2. Acquisition of recombinant bacteria

[0061] The recombinant vectors pSuper1300-ZmCPS1 and pSuper1300-K409Y ZmCPS1 were respectively introduced into Agrobacterium tumefaciens GV3101 to obtain recombinant bacteria, which were named GV3101-pSuper1300-ZmCPS1 and GV3101-pSuper1300-K409Y ZmCPS...

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Abstract

The invention discloses a method and application for improving the sensitivity of monocotyledonous crops to a gibberellin inhibitor. Protein is provided and is a protein mutant obtained by mutating lysine at the position 409 of the amino acid sequence of the protein ZmCPS1 into tyrosine. Experiments prove that lysine (K) at the position 409 of ZmCPS1 is edited as tyrosine (Y) to obtain the K409YZmCPS1 mutant which is transferred into corn so that the sensitivity of the corn to mepiquat chloride can be changed, the economical and safe gibberellin synthesis inhibitor, namely mepiquat chloride isapplied to corn cultivation to achieve effective shaping of ideal plant types of the corn, and finally the effects of increasing the yield and improving the resistance of the corn are achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and application for improving the sensitivity of monocot crops to gibberellin inhibitors. Background technique [0002] Corn (Zea mays L.) is a very important food crop, feed crop, industrial raw material and energy crop in the world. It plays an irreplaceable role in the development of the national economy. The two countries with the largest corn production in the world are the United States and China (Ci et al., 2012). But at present, due to factors such as changes in climatic conditions and unreasonable irrigation, the phenomenon of corn lodging is becoming more and more serious, which makes it difficult to harvest corn and seriously affects its yield. Therefore, it is undoubtedly possible to solve the problem of lodging by shaping the plant type of corn. First choice. In order to increase the yield of maize, it is possible to inhibit plant growth and internode shorteni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50C12N15/57C12N15/82
Inventor 李召虎张娟张钰石张明才邢嘉鹏
Owner CHINA AGRI UNIV