Seedling cultivation method of radermachera sinica

A cultivating method and technology for Vitiligo, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of unfavorable protection of wild unicornis germplasm resources, over-exploitation of unicornis, difficulty in seed collection, etc. Achieve rapid breeding and cultivation, good leaf color and development status, good growth and development status

Active Publication Date: 2019-05-17
SICHUAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some individual nurseries excavate wild Kylin Bignonia from the mountains for sale as fake plants, resulting in the overexploitation of wild Kylin Bignonia, which is very detrimental to the protection of wild Kylin Bignonia germplasm resources
At present, Kylin Bignonia is mainly propagated by sowing or cuttings, but because its seeds are easy to crack, it is difficult to collect seeds, which in turn affects the price and is susceptible to fluctuations due to the impact of seed supply. The procedure is cumbersome and the survival rate is low

Method used

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  • Seedling cultivation method of radermachera sinica
  • Seedling cultivation method of radermachera sinica

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Embodiment 1

[0032] The seedling cultivation method of Kylin Bignonia described in the present embodiment may further comprise the steps:

[0033] (1) Disinfection of explants: select the freshly pumped tender stems (with 5-6 nodes) of the two-year-old Kylin Bignonia tree as explants for tissue culture, and cut the tender stems of Kylin Bignonia with leaves removed into strips. For a small section with 2-3 nodes, wash it under running water for 0.5h, and then soak it in 75% alcohol for 30s and then 0.05% HgCl on the ultra-clean workbench. 2 Soak in the solution for 10 minutes, and rinse with sterile water 4-5 times to complete the disinfection of the explants, and set aside;

[0034] (2) Bud induction and cultivation: the two ends of the sterilized Kylin Bignonia tender stems are respectively excised a part, all cut into 1.0cm, a segment with a node, insert the above-mentioned MS nutrient solution as the basic medium, and Add chitosan oligosaccharide (content 8g / L) and jasmonate (content ...

Embodiment 2

[0039] The seedling cultivation method of Kylin Bignonia described in the present embodiment may further comprise the steps:

[0040] (1) Disinfection of explants: select the freshly pumped tender stems (with 5-6 nodes) of the two-year-old Kylin Bignonia tree as explants for tissue culture, and cut the tender stems of Kylin Bignonia with leaves removed into strips. For a small section with 2-3 nodes, wash it under running water for 0.5h first, then soak it in 75% alcohol for 60s and then 0.05% HgCl on the ultra-clean workbench 2 Soak in the solution for 8 minutes, and rinse with sterile water 4-5 times to complete the disinfection of the explants, and set aside;

[0041] (2) Bud induction and cultivation: the two ends of the sterilized Kylin Bignonia tender stems are respectively excised a part, all cut into 1.0cm, a segment with a node, insert the above-mentioned MS nutrient solution as the basic medium, and Add chitosan oligosaccharide (content 12g / L) and jasmonate (content...

Embodiment 3

[0046] The seedling cultivation method of Kylin Bignonia described in the present embodiment may further comprise the steps:

[0047] (1) Disinfection of explants: select the freshly pumped tender stems (with 5-6 nodes) of the two-year-old Kylin Bignonia tree as explants for tissue culture, and cut the tender stems of Kylin Bignonia with leaves removed into strips. For a small section with 2-3 nodes, wash it under running water for 0.5h first, then soak it in 75% alcohol for 45s and then 0.05% HgCl on the ultra-clean workbench 2 Soak in the solution for 9 minutes, and rinse with sterile water 4-5 times to complete the disinfection of the explants, and set aside;

[0048] (2) Bud induction and cultivation: the two ends of the sterilized Kylin Bignonia tender stems are respectively excised a part, all cut into 1.0cm, a segment with a node, insert the above-mentioned MS nutrient solution as the basic medium, and Add chitooligosaccharide (content 10g / L) and jasmonate (content 1mg...

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Abstract

The invention belongs to the technical field of seedling cultivation and particularly relates to a seedling cultivation method of radermachera sinica. According to the seedling cultivation method of the radermachera sinica, on the basis of the existing radermachera sinica seedling in-vitro tissue cultivation mode and through adjustment of a bud induction culture medium, a bud proliferation culturemedium and a rooting culture medium, good growing condition of the radermachera sinica seedlings is guaranteed, a large amount of high-quality seeds and seedlings are provided for the market, particularly each cultivation period of the radermachera sinica seedlings is greatly shortened, and rapid breeding cultivation of the radermachera sinica is realized.

Description

technical field [0001] The invention belongs to the technical field of seedling cultivation, and in particular relates to a method for cultivating unicorn bignonia seedlings. Background technique [0002] Kylin Bignonia, scientific name Bean tree, alias pepper tree, elder parasol, mountain bean tree or happiness tree, belongs to Bignoniaceae, Bean tree genus, flowering period is May-September, fruit period is October-December. Kylin Bignonia is light and tough, not easy to deform and warp, and can be used as materials for construction, furniture, and vehicles; its roots, leaves, and fruits can be used as medicine, cooling blood, reducing swelling, and treating high fever, bruises, snake bites, etc. Efficacy; its branches, leaves and roots can cure cattle anthracnose. The Kylin Bignonia potted plant is beautiful in shape, with small and emerald green leaves and whirling shadows. It is a very precious indoor ornamental plant. As a potted ornamental tree, Kirin Bignonia is mo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周静曾学英张炜
Owner SICHUAN NORMAL UNIVERSITY
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