Application of clozapine in delaying senescence of cultured mesenchymal stem cells

A technology of mesenchymal stem cells and clozapine, applied in the field of cell culture, can solve the problems of high cost and unstable ingredients, and achieve the effect of low cost and no toxic effect

Active Publication Date: 2019-05-17
广州瑞铂茵健康科技有限公司
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Problems solved by technology

[0005] There are some deficiencies in the current reagents used to delay the aging of MSCs: 1) the cost is high, such as growth factors and nutrients; 2) the ingredients are unstable and often contain different types of impurities, which will bring a series of problems for clinical-grade MSCs. Predicted risks, such as unicellular algae extract

Method used

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  • Application of clozapine in delaying senescence of cultured mesenchymal stem cells
  • Application of clozapine in delaying senescence of cultured mesenchymal stem cells
  • Application of clozapine in delaying senescence of cultured mesenchymal stem cells

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Embodiment Construction

[0022] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0023] Umbilical cord mesenchymal stem cell preparation

[0024] 1) Wash the umbilical cord with sterile saline, cut it into small pieces, put it into an empty petri dish, tear the umbilical cord with tissue forceps, peel off the two arteries, and tear off the Walton's glue distributed around the blood vessels ;Put the separated Walton's jelly into a 50mL centrifuge tube, add 2mL of medium, cut the Walton's jelly into 1mm pieces with scissors, then add 6mL of primary medium, mix well Put into 6 T25 culture flasks;

[0025] 2) Put the T25 culture bottle into the incubator at 37°C, 5% CO 2 to cultivate;

[0026] 3) When 2 to 3 colonies grow in each T25 culture flask, and each colony has at least hundreds of cells, pour out the medium and tissu...

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Abstract

The invention discloses application of clozapine in delaying senescence of cultured mesenchymal stem cells, and belongs to the technical field of cell culture. A clozapine diluent is more convenient,safer and more efficient to use in the process of delaying the senescence of the mesenchymal stem cells, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to the application of clozapine in delaying the senescence of cultured mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) have strong self-renewal and multi-lineage differentiation potential, and have been widely used in the fields of tissue engineering and regenerative medicine. At present, after extensive research, MSCs can differentiate into osteoblasts, chondrocytes, adipocytes, nerve cells, etc., which have broad prospects for regenerative medicine applications. [0003] In order to obtain a sufficient number of MSCs for clinical treatment, MSCs need to be expanded in vitro, but replicative senescence inevitably occurs during the subculture and proliferation of stem cells. In order to slow down the aging of MSCs produced during the culture process and better maintain the characteristics of stem cells, scientific research teams ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 嵐山芮魏伟李祎
Owner 广州瑞铂茵健康科技有限公司
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