Microbial network-based method for assessing risk of environmental pollution

A technology of network evaluation and microorganisms, applied in the fields of biological systems, bioinformatics, informatics, etc., can solve the problems of microorganisms that do not consider the influence of environmental factors, ignore the coexistence information of microorganisms, etc., and achieve the effect of accurate judgment

Active Publication Date: 2019-05-21
TSINGHUA UNIV
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  • Abstract
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Problems solved by technology

However, these methods do not consider the influence of environmental factors when estimating the association between microorganisms, and ignore the hidden microbial coexistence information in the construction of a larger interaction network between environmental factors and microbial communities.

Method used

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  • Microbial network-based method for assessing risk of environmental pollution
  • Microbial network-based method for assessing risk of environmental pollution
  • Microbial network-based method for assessing risk of environmental pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Example: Bacterial community and basic environmental indicators in the sediment samples of the same river network

[0024] refer to figure 1 The flow chart is used to construct the interaction relationship between microorganisms and environmental factors, and compare the changes in the coexistence relationship of microorganisms to predict and verify the risk of environmental pollution. The specific operation steps are as follows:

[0025] Select 5 sampling points in the sediment of the same river in the middle reaches of a certain river for sampling, and measure the basic indicators of carbon, nitrogen and phosphorus and metal pollution indicators. The results show that the nitrogen and phosphorus indicators in the sediment samples are normal, and the metal and other indicators are not beyond the national pollution risk control standard. DNA was extracted from the sample using metagenomic sequencing, and a clustering table based on similarity was obtained through bioi...

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Abstract

The invention relates to a microbial network-based method for assessing the risk of environmental pollution. The method is mainly used for assessing the risk of pollution of environmental media such as soil, surface water, underground water and bottom mud. The method comprises the following steps: obtaining microbial community structure and relative abundance data of an environmental sample through metagenome sequencing, and obtaining basic environmental indexes of the corresponding sample at the same time; and constructing a microorganism interaction network model according to the dominant microorganisms and the key environment indexes. The stability of the environmental micro-ecosystem is evaluated through the positive and negative correlation ratio and the network average degree changein the microorganism coexistence relationship. The negative correlation ratio is reduced to indicate that the environmental pollution risk is increased. Proper environmental pollution prevention measures need to be taken. Compared with a conventional method for monitoring and evaluating environmental pollution based on a plurality of environmental physicochemical indexes, the environmental pollution monitoring and evaluating method based on the ecological statistics can make a pre-judgment before the occurrence of pollution, and is helpful for a monitor to make a timely and accurate judgment on the environmental pollution prevention and control opportunity.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution assessment, in particular to a method for assessing environmental pollution risks based on microbial networks. Background technique [0002] Environmental pollution refers to harmful substances that pollute the atmosphere, water, soil, animals and plants to a harmful degree (physical pollution, chemical pollution, biological pollution, etc.). Usually, my country monitors and controls pollution by formulating pollution risk management and control standards for different environmental media. To prevent environmental pollution, the basic indicators such as cadmium, mercury, arsenic, and lead are used as the basic items of the soil pollution risk screening value of agricultural land, and the total amount of HCH, the total amount of DDT and benzo[a]pyrene are selected as pollutants to be measured project to assess the risk of soil contamination on agricultural land. However, due to the w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B5/00
Inventor 张芳魏雨泉李广贺张昊郑迪张旭
Owner TSINGHUA UNIV
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