A Method for Assessing Environmental Pollution Risk Based on Microbial Network

A network assessment and microbial technology, applied in biological systems, bioinformatics, instruments, etc., can solve the problems of microorganisms not considering the influence of environmental factors, ignoring the coexistence information of microorganisms, etc., and achieve the effect of accurate judgment

Active Publication Date: 2021-03-16
TSINGHUA UNIV
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Problems solved by technology

However, these methods do not consider the influence of environmental factors when estimating the association between microorganisms, and ignore the hidden microbial coexistence information in the construction of a larger interaction network between environmental factors and microbial communities.

Method used

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  • A Method for Assessing Environmental Pollution Risk Based on Microbial Network
  • A Method for Assessing Environmental Pollution Risk Based on Microbial Network
  • A Method for Assessing Environmental Pollution Risk Based on Microbial Network

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Example: Bacterial community and basic environmental indicators in sediment samples from the same river network

[0024] refer to figure 1 The flow chart of the microbial-environmental factor interaction relationship is constructed, and the changes in the microbial coexistence relationship are compared, and the environmental pollution risk is predicted and verified. The specific operation steps are as follows:

[0025] Five sampling points in the sediment of the same section in the middle reaches of a river were selected for sampling, and the basic indicators of carbon, nitrogen and phosphorus and metal pollution indicators were determined. The results showed that the indicators of nitrogen and phosphorus in the sediment samples were normal, and the indicators such as metals did not exceed the national pollution risk control and control. standard. DNA was extracted from the samples using metagenomic sequencing, and bioinformatics analysis was used to obtain a clusteri...

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Abstract

The invention relates to a microbial network-based method for assessing the risk of environmental pollution. The method is mainly used for assessing the risk of pollution of environmental media such as soil, surface water, underground water and bottom mud. The method comprises the following steps: obtaining microbial community structure and relative abundance data of an environmental sample through metagenome sequencing, and obtaining basic environmental indexes of the corresponding sample at the same time; and constructing a microorganism interaction network model according to the dominant microorganisms and the key environment indexes. The stability of the environmental micro-ecosystem is evaluated through the positive and negative correlation ratio and the network average degree changein the microorganism coexistence relationship. The negative correlation ratio is reduced to indicate that the environmental pollution risk is increased. Proper environmental pollution prevention measures need to be taken. Compared with a conventional method for monitoring and evaluating environmental pollution based on a plurality of environmental physicochemical indexes, the environmental pollution monitoring and evaluating method based on the ecological statistics can make a pre-judgment before the occurrence of pollution, and is helpful for a monitor to make a timely and accurate judgment on the environmental pollution prevention and control opportunity.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution assessment, and in particular relates to a method for assessing environmental pollution risk based on a microbial network. Background technique [0002] Environmental pollution refers to the pollution of harmful substances to the atmosphere, water, soil, animals and plants to a harmful degree (physical pollution, chemical pollution, biological pollution, etc.) To prevent environmental pollution, the basic indicators such as cadmium, mercury, arsenic, and lead are used as the basic items for the screening value of soil pollution risk in agricultural land, and the total amount of hexahexanol, the total amount of DDT and benzo[a]pyrene are selected as the selected pollutants. project to assess soil contamination risk in agricultural land. However, due to the wide variety of pollutants existing in various environmental media, pollution risk assessment based on existing characteristic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B5/00
Inventor 张芳魏雨泉李广贺张昊郑迪张旭
Owner TSINGHUA UNIV
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