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Application of ramulus et folium picrasmae extract to preparing medicines or healthcare products for preventing and treating neurodegenerative diseases

A neurodegenerative, bitterwood extract technology, applied in the application field of bitterwood extract in the preparation of drugs or health care products for the prevention and treatment of neurodegenerative diseases, can solve the problems that neuroprotection has not been reported yet

Inactive Publication Date: 2019-05-24
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bitterwood and its β -The neuroprotective effect of carbalin alkaloids has not been reported

Method used

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  • Application of ramulus et folium picrasmae extract to preparing medicines or healthcare products for preventing and treating neurodegenerative diseases
  • Application of ramulus et folium picrasmae extract to preparing medicines or healthcare products for preventing and treating neurodegenerative diseases
  • Application of ramulus et folium picrasmae extract to preparing medicines or healthcare products for preventing and treating neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Different extracts of bitter wood and three β - Preparation of carbalin alkaloids

[0027] 1. Medicinal materials and reagents

[0028] Bitterwood stems were collected in Ganzhou, Jiangxi, China in August 2016. Reagents such as ethanol, dichloromethane, and methanol were all analytically pure.

[0029]2. Extraction and separation

[0030] 20 kg of dried stems of Bitterwood, sliced, extracted three times with 80% ethanol under reflux for two hours each time, concentrated the extract in vacuo, and combined to obtain 290 g of the total extract. The total extract was dispersed in water, extracted three times with equal volumes of ethyl acetate and n-butanol respectively, and after recovering the solvent, 64 g of ethyl acetate extract (PQE), 78 g of n-butanol extract (PQB) and water Extract (PQW) 130 g. PQE was subjected to silica gel column chromatography, and CH 2 Cl 2 -MeOH (50:1−50:50) was eluted as the eluent, and a total of 8 fractions (Fr.1−8) were ob...

Embodiment 2

[0035] Implementation 2 In vitro experiment of different extracts of bitter wood

[0036] 1. Experimental materials

[0037] Cells: PC12 cell line and SH-SY5Y cell line are all from Shanghai Cell Bank, Chinese Academy of Sciences.

[0038] Drugs: different extracts of bitter wood (PQE, PQB and PQW); L - Glutamic acid was purchased from Shanghai Huixing Biochemical Reagent Co., Ltd.; β - Amyloid 25-35 (A β 25-35 ) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China.

[0039] A β 25-35 Stock solution preparation: 1 mg A β 25-35 Soluble in 500 mu L normal saline, incubate at 37 °C incubator for 7 days, store in -20 °C refrigerator, and set aside.

[0040] 2. Cell Culture

[0041] Both PC12 cell line and SH-SY5Y cell line were cultured in DMEM incomplete high-glucose medium containing 10% fetal bovine serum, CO 2 The culture conditions in the cell incubator were 37 °C, 5% CO 2 Concentration, saturated humidity, after the cells grow ...

Embodiment 3

[0052] Implement 3 PQE in vivo anti-AD experiments

[0053] 1. Materials

[0054] 1.1 Experimental animals and grouping

[0055] 42 healthy male ICR mice, weighing 18-22 g, were provided by Qinglongshan Animal Breeding Farm, Jiangning District, Nanjing, license number: SCXK (Su) 2017-0001. Randomly divided into sham operation group, A β 25-35 Model group, A β 25-35 + 25 mg / kg PQE group (low dose group), A β 25-35 + 50 mg / kg PQE group (middle dose group), A β 25-35 + 100 mg / kg PQE group (high dose group), A β 25-35 + 1.3 mg / kg donepezil group (positive group) each with 7 rats.

[0056] 1.2 Experimental reagents and main equipment

[0057] Shilepu (Donepezil Hydrochloride Tablets)

Jiangsu Hansoh Co., Ltd.

Paraformaldehyde

Sinopharm Group Industry Co., Ltd.

β - Amyloid 25-35

China Shanghai Aladdin Biochemical Technology Co., Ltd.

BCA protein quantification kit

Shanghai Biyuntian Biotechnology Co., Ltd.

mouse ...

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PUM

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Abstract

The invention discloses application of ramulus et folium picrasmae extract to preparing medicines or healthcare products for preventing and treating neurodegenerative diseases. The ramulus et folium picrasmae extract comprises ramulus et folium picrasmae ethyl acetate extract and / or beta-carboline alkaloid separated from ramulus et folium picrasmae. The neurodegenerative diseases include Alzheimer's diseases, Parkinson's diseases, dementia with Lewy bodies, frontotemporal dementia, Huntington's diseases and amyotrophic lateral sclerosis. The application has the advantage that novel treatment pathways further can be provided to preventing and / or treating the neurodegenerative diseases.

Description

technical field [0001] The invention belongs to the technical field of medicaments and health care products, and in particular relates to the application of bitterwood extracts in the preparation of medicines or health care products for preventing and treating neurodegenerative diseases. Background technique [0002] Neurodegenerative diseases are characterized by the loss of a large number of specific neurons. They are progressive, disabling, severe and fatal complex diseases, mainly including Alzheimer's disease and Huntington's disease. , Parkinson's disease, Lewy body dementia, frontotemporal dementia and amyotrophic lateral sclerosis, etc. This kind of disease is widely spread in the world, with a long duration and lack of effective treatment, which brings a heavy burden to individuals, families and society. Only a single subtype of Alzheimer's disease (Alzheimer disease, AD) in the elderly The cause of death ranks fourth after cancer, heart disease, and cerebrovascula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P25/28A61P25/16A61P25/14C07D471/04A61K31/437A23L33/105
Inventor 柳文媛徐健郭二燕冯锋曲玮张杰孙昊鹏朱慧琳胡韫伟
Owner CHINA PHARM UNIV
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