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A molecular marker for detecting scab resistance qtl Qfhb.hbaas-5AS and its application method

A technology for scab and wheat scab, which is applied in the field of molecular markers for detecting scab-resistance QTLQfhb.hbaas-5AS, which can solve the problem of less application of gene breeding and the like

Active Publication Date: 2022-06-21
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, except for Fhb1, the breeding applications of other genes are less reported

Method used

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  • A molecular marker for detecting scab resistance qtl Qfhb.hbaas-5AS and its application method
  • A molecular marker for detecting scab resistance qtl Qfhb.hbaas-5AS and its application method
  • A molecular marker for detecting scab resistance qtl Qfhb.hbaas-5AS and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Discovery of Qfhb.hbaas-5AS and its associated SNP markers

[0043] Test material: WAPS (Wheat Association Panel for Scab research) group formed by 240 wheat varieties (lines) at home and abroad, see Table 1. The materials used are recorded in the literature: Zhu Zhanzhang, Xu Dengan, Cheng Shunhe, et al. Identification and traceability of the stripe rust resistance gene Fhb1 in Chinese wheat varieties [J]. Journal of Crops, 2018, 44(4): 473–482. The public can download from Hubei Obtained from the Institute of Food Crops, Provincial Academy of Agricultural Sciences.

[0044] Table 1 Varieties (lines) of WAPS population and their sources

[0045]

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[0048]

[0049]

[0050]

[0051]

[0052]

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[0054] a 90K SNP chip typing results; b For KASP marker detection results; c FHB index BLUE value (Best linear unbiased estimate) in 4 environments, "NN" is missing chip data.

[0055] 1. Discovery of Qfhb.hbaas-5A...

Embodiment 2

[0099] Example 2. Actual sample detection

[0100] Test materials: WAPS population varieties shown in Table 1.

[0101] 1. Identification of resistance to scab

[0102] Same as Example 1.

[0103] 2. Detection of KASP-Fhb5AS marker

[0104] Marking with KASP-Fhb5AS According to the method in Example 1, KASP reaction was carried out on the 240 varieties shown in Table 1, and KASP reaction products were detected; Plus Detect the product, and use KlusterCaller software for data analysis; if the color is red, the genotype of the wheat SNP site IWB21456 to be tested is AA; if it is blue, the gene of the wheat SNP site IWB21456 to be tested is Type is GG.

[0105]Table 1 shows the marker detection results and the average value of FHB index of 240 wheat varieties. Statistical analysis was performed on the results in Table 1, and the results in Table 4 were obtained.

[0106] Table 4 Differences in resistance to Fusarium head blight among different genotypes of KASP-Fhb5AS

[0...

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Abstract

The invention discloses a molecular marker for detecting scab resistance QTL Qfhb.hbaas‑5AS and a method for using it. The present invention provides the application of the genotype detection material of the SNP IWB21456 site of wheat chromosome 5AS in identification or auxiliary identification of wheat head blight resistance; also provides the detection of the genotype material of the SNP IWB21456 site of wheat chromosome 5AS Application in preparation and identification or auxiliary identification of wheat head blight resistance products. The present invention discovers the head blight resistance locus Qfhb.hbaas-5AS located on the short arm of the wheat 5A chromosome through genome-wide association analysis (GWAS), and further converts its associated SNP IWB21456 into a common PCR marker KASP-Fhb5AS, and the two pair 240 The type agreement rate of each material was 96.7%. This marker can be used to detect the genotype of QTL Qfhb.hbaas‑5AS for resistance to head blight and to be used in molecular breeding for resistance to head blight.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker for detecting the anti-head blight QTL Qfhb.hbaas-5AS and a method for using the same. Background technique [0002] Fusarium head blight (FHB), caused by Fusarium graminearum, is a widespread fungal disease that seriously affects the yield and quality of wheat. More importantly, susceptible grains contain mycotoxins, such as Deoxynivalenol (DON), which endanger human and animal health and affect food and feed. [0003] The middle and lower reaches of the Yangtze River and the Northeast wheat area of ​​my country are the areas where the wheat scab is often and recurring. In recent years, affected by global climate change and the popularization of straw returning under the wheat-maize rotation system, the disease has been expanding and aggravating, and has become a common disease in the Huanghuai wheat area. At present, the average annual occurrence area of ​​whea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 朱展望付路平陈延平刘易科何伟杰贾梦洁郝元峰高春保夏先春何中虎
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI