A kind of fluorescent OCT dual-mode imaging method and device based on indocyanine green nanometers

Abstract

A fluorescence-OCT dual-mode imaging method and device based on indocyanine green nanometers, the method comprising: combining indocyanine green with phospholipidated polyethylene glycol to form indocyanine green-phospholipidated polyethylene glycol Nanoparticles, and modify biological targeting molecules at the end of the polyethylene glycol molecule to obtain ICG-PL-PEG-FA solution; inject the ICG-PL-PEG-FA solution to the imaging target, and use the low coherence of near-infrared light The light source irradiates the area to be scanned of the imaging target and uses a light source with a wavelength of 780nm to excite the ICG-PL-PEG-FA molecules in the imaging target to generate fluorescence; open the OCT imaging optical path to obtain the OCT imaging of the imaging target Structural images with depth ranges. The above method and device generate fluorescence through the ICG-PL-PEG-FA molecules in the imaging target, and use the OCT imaging system to collect the fluorescence signals, which can restore the position information of the fluorescent substance in the experimental living body, so that the contrast agent can be clearly distinguished The position of the identified lesion area presents a structural image of a certain depth range.

Description

technical field [0001] The invention belongs to the field of biological imaging, in particular to a fluorescence-OCT dual-mode imaging method and device based on indocyanine green nanometers. Background technique [0002] Optical coherence tomography (OCT) and fluorescence imaging technology are widely used in medical diagnosis. OCT technology uses the basic principle of weak coherent light interference to detect the back reflection or several times of incident weak coherent light at different depths of biological tissue. Scattering signals, through scanning, can obtain biological tissue depth signals, reconstruct two-dimensional or three-dimensional structural images, and thus obtain biological depth information. Fluorescence imaging technology uses fluorescent probes to generate and collect fluorescent signals by exciting the probes with an excitation light source. A single program mode cannot obtain comprehensive functional and structural imaging information to understan...

AI Technical Summary

Problems solved by technology

However, at present, ICG imaging is generally realized by using fluorescence imaging technology, and fluorescence imaging only obtains the surface information of the detection area, and cannot explore the shortcomings of the depth direction signal.

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