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Uric acid kit for rapidly and simply eliminating drug interference of calcium dobesilate and etamsylate in serum

一种羟苯磺酸钙、酚磺乙胺的技术，应用在医学检验领域，能够解决负偏差、消耗尿酸值、医师诊断治疗误判等问题，达到消除负干扰、准确度高、精准用药及治疗的效果

Active Publication Date: 2021-03-19
ZHONGSHAN BGH BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Calcium dobesilate is used as a commonly used drug for microvascular protection and treatment, and fensulfate is used as a commonly used drug for hemostasis. These drugs are all strong reducing agents. When patients taking these drugs measure plasma uric acid levels, these reducing drugs will H produced by the decomposition of uric acid by uricase 2 o 2 direct reaction, consumes H 2 o 2 Cause serious negative deviation of uric acid value, resulting in misjudgment of doctors in diagnosis and treatment

Method used

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  • Uric acid kit for rapidly and simply eliminating drug interference of calcium dobesilate and etamsylate in serum
  • Uric acid kit for rapidly and simply eliminating drug interference of calcium dobesilate and etamsylate in serum
  • Uric acid kit for rapidly and simply eliminating drug interference of calcium dobesilate and etamsylate in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Reagent R1 components:

[0065] Tris buffer 50mM pH8.0;

[0066] Peroxidase 9ku / L;

[0067] Ascorbate oxidase 6ku / L;

[0068] Vanadium pentoxide 1mM;

[0069] 4-AAP 3mM;

[0070] TRITON X-100 0.2%;

[0071] Borax 1%

[0072] Reagent R2 components:

[0073] Tris buffer 50mM pH8.0

[0074] Uricase 9ku / L

[0075] Bovine Serum Albumin 0.1%

[0076] TOOS 10mM

[0077] Borax 1.0%

[0078] TRITON 0.1%.

Embodiment 2

[0080] Reagent R1 components:

[0081] HEPES buffer 50mM pH8.0

[0082] Peroxidase 7.2ku / L

[0083] Ascorbate Oxidase 6.4ku / L

[0084] Sodium ferrate 1mM

[0085] 4-AAP 3mM

[0086] TRITON X-100 0.2%

[0087] Gentamicin Sulfate 0.1%;

[0088] Reagent R2 components:

[0089] HEPES buffer 50mM pH8.0

[0090] Uricase 11.2ku / L

[0091] Bovine Serum Albumin 0.1%

[0092] TOOS 10mM

[0093] Gentamicin Sulfate 0.1%

[0094] TRITON 0.1%.

Embodiment 3

[0096] Reagent R1 components:

[0097] Phosphate buffer 50mM pH8.0

[0098] Peroxidase 4.6ku / L

[0099] Ascorbate Oxidase 10ku / L

[0100] Sodium metavanadate 2mM

[0101] 4-AAP 3mM

[0102] TRITON X-100 0.2%

[0103] Sodium azide 0.1%;

[0104] Reagent R2 components:

[0105] Phosphate buffer 50mM pH8.0

[0106] Uricase 20ku / L

[0107] Bovine Serum Albumin 0.1%

[0108] TOOS 10mM

[0109] Sodium Azide 0.1%

[0110] TRITON X-100 0.1%.

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Abstract

The invention relates to a uric acid kit for rapidly and simply eliminating drug interference of calcium dobesilate and etamsylate in serum. The uric acid kit is characterized by comprising a reagentR1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, creatinase, creatine oxidase, ascorbic acid oxidase, peroxidase or catalase, serum albumin, a nonionic surfactant, a preservative, a Trinder's reactant A and laccase or metallate; and the reagent R2 comprises a buffer solution, serum albumin, creatininase, a preservative and a Trinder's reactant B. According to the invention,the defect that creatinine determination is inaccurate due to negative deviation caused by the calcium dobesilate and the etamsylate contained in serum samples when creatinine is determined by clinical examination in various hospitals at present is overcome, and the uric acid kit is suitable for detection of serum creatinine by a full-automatic biochemical analyzer.

Description

[0001] This case is a divisional application, specifically the application number: 2020108215098, and the title is: A uric acid kit and its preparation method that can eliminate the interference of calcium dobesilate and phensulfonate in serum, and the application date is: 2020.08.15 divisional application. technical field [0002] The invention belongs to the technical field of medical testing, and relates to a uric acid test kit for quickly and simply eliminating the interference of calcium dobesilate and ethanolamine in serum. Background technique [0003] Uric acid (Uric Acid, UA) is the final product of the metabolism of purine compounds in the body, which is weakly acidic. Uric acid can come from the body or from the catabolism of purines in food. In the body, uric acid is mainly produced in the liver, a small part can be excreted through the liver with bile, and most of the rest are excreted from the kidneys. The solubility of uric acid is small, so it is easy to pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/62C12Q1/28C12Q1/26
CPCC12Q1/62C12Q1/28C12Q1/26G01N2333/90235G01N2333/90694C12Q2326/96G01N2333/902Y02A50/30
Inventor 俞永标
Owner ZHONGSHAN BGH BIOTECH CO LTD
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