An electrochemical measurement method

A measurement method and electrochemical technology, applied in the field of electrochemical measurement, can solve the problems of complex manufacturing of test strips, weak anti-interference ability, and lack of methods to effectively eliminate the negative interference of detection reduction current, so as to achieve a wide range of applications and anti-interference ability. Strong, eliminate negative interference effect

Active Publication Date: 2017-03-15
SINOCARE
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although the above method has been partially successfully applied to the interference elimination of electrochemical test strips, it also has disadvantages such as weak anti-interference ability and complicated test strip manufacturing.
In addition, the current anti-interference methods are only used to reduce the positive interference when the oxidation current is detected, and there is no method to effectively eliminate the negative interference when the reduction current is detected. Therefore, the development of new methods can reduce the positive interference caused by the reducing interference in the electrochemical test strip The general approach to interference or negative interference has significant

Method used

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Embodiment 1

[0047] Embodiment 1. Reduce the positive interference of reducing interfering substances on the glucose electrochemical sensor with oxidation current as the detection signal.

[0048] The first working electrode 11 is coated with glucose oxidase, oxidized electron mediator (potassium ferricyanide), polymer adhesion promoter (hydroxyethyl cellulose) and filler (lactose), and the second working electrode 13 and reference The specific electrode 12 is coated with an oxidized electron mediator (potassium ferricyanide), a polymer adhesion promoter (hydroxyethyl cellulose) and a filler (lactose). Link the electrochemical test strip with the potentiostatic measuring instrument (CHI electrochemical workstation, Shanghai Chenhua), and apply a positive voltage of 0.3V relative to the reference electrode 12 on the first working electrode 11 and the second working electrode 13 simultaneously, then The current I related to the glucose concentration is measured on the surface of the first wo...

Embodiment 2

[0055] Embodiment 2, reducing the negative interference of reducing interfering substances on the glucose electrochemical sensor with reducing current as the detection signal.

[0056] The structure of the electrochemical test strip used in Example 2 is the same as that in Example 1.

[0057] The first working electrode 11 is coated with glucose oxidase, peroxidase, reduced type electron mediator (potassium ferrocyanide), polymer adhesion promoter (hydroxyethyl cellulose) and filler (lactose), The second working electrode 13 is coated with novel ascorbate oxidase, peroxidase, reduced electron mediator (potassium ferrocyanide) and macromolecule adhesion promoter (hydroxyethyl cellulose) that catalyze ascorbic acid to produce hydrogen peroxide and filled with agent (lactose), and the reference electrode 12 is coated with a reduced electron mediator (potassium ferrocyanide), a polymer adhesion promoter (hydroxyethyl cellulose) and a filler (lactose). The electrochemical test str...

Embodiment 3

[0064] Embodiment 3, reducing the negative interference of reducing interfering substances to the blood lipid electrochemical sensor with reducing current as the detection signal

[0065] The structure of the electrochemical test strip used in embodiment 3 is the same as that in embodiment 1.

[0066] The first working electrode 11 is coated with cholesterol esterase, cholesterol oxidase, peroxidase, reduced electron mediator (potassium ferrocyanide), polymer adhesion promoter (hydroxyethyl cellulose) and filler ( lactose), the second working electrode 13 is coated with novel ascorbate oxidase, peroxidase, reduced electron mediator (potassium ferrocyanide) and polymer adhesion promoter (hydroxyethyl cellulose) that catalyze ascorbic acid to produce hydrogen peroxide ) and a filler (lactose), the reference electrode 12 is coated with a reduced electron mediator (potassium ferrocyanide), a polymer adhesion promoter (hydroxyethyl cellulose) and a filler (lactose). The electroche...

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Abstract

The invention belongs to the technical field of electrochemical measurement methods and discloses an electrochemical measurement method which can be used for alleviating influence of reducing interferent in an electrochemical test strip. The electrochemical measurement method comprises the following steps: respectively measuring a current signal I1 generated by a target analyte and an interference current signal I2 generated by a reducing interferent, calculating corresponding correction factors k according to the current signal I1 generated by the target analyte and the interference current signal I2 generated by the reducing interferent, and correcting the current signal generated by the target analyte by utilizing the correction factors k to obtain a real correction current value Ic corresponding to the real concentration of the target analyte, wherein Ic is equal to I1 / k. The measurement method provided by the invention can be used for effectively alleviating influence on the measurement result caused by the reducing interferent, not only is suitable for reducing positive interference during detection of the oxidation current, but also can be used for effectively eliminating negative interference during detection of the reduction current, and is high in measurement accuracy, strong in anti-interference ability and wide in application range.

Description

technical field [0001] The invention belongs to the technical field of electrochemical measurement, in particular to an electrochemical measurement method, in particular to an electrochemical measurement method for reducing the influence of reducing interfering substances in electrochemical test strips. Background technique [0002] Electrochemical measurement systems based on the principle of electrochemical enzymatic amperometric biosensors are commonly used in various point-of-care testing (POCT) products, such as commercialized electrochemical measuring instruments for blood glucose, blood ketones, blood lipids, and blood creatinine. The electrochemical measurement system realizes the rapid quantitative determination of the target analyte by detecting the enzyme-catalyzed oxidation or reduction current of the target analyte. For example, electrochemical measuring instruments for blood glucose (or blood ketone) often use oxidase (or dehydrogenase-coenzyme) and oxidized el...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 黄钊蔡晓华
Owner SINOCARE
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