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A kind of alkaloid compound and its preparation method and application

A compound and alkaloid technology, applied in the field of alkaloid compounds and their preparation, can solve the problems of high mortality, failure to improve the survival period of glioma patients, and easy recurrence of glioma, and achieve the effect of broad application prospects

Active Publication Date: 2021-09-28
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relevant studies have shown that the addition of temozolomide to radiotherapy can improve the survival of patients with glioma. Drug resistance, etc., have brought great challenges to the treatment of glioma. Glioma is prone to recurrence, high mortality rate, and the treatment effect is not optimistic. Bevacizumab, which has the effect of inhibiting vascular endothelial growth factor , belongs to anti-tumor angiogenesis therapy, but studies have shown that bevacizumab cannot improve the survival of glioma patients, and not all glioma patients are sensitive to bevacizumab

Method used

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  • A kind of alkaloid compound and its preparation method and application
  • A kind of alkaloid compound and its preparation method and application
  • A kind of alkaloid compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The acquisition and characterization of the compound of Example 1

[0032] 1. The specific preparation steps of the compound are as follows:

[0033] S1. Preparation of seed culture medium

[0034] S11. Prepare seed medium:

[0035] The components and dosage of the seed medium are: 200 g of potatoes, 20 g of glucose, and 1 L of deionized water. After preparation, they are evenly distributed in five 500 mL Erlenmeyer flasks, and extinguished at 121° C. for 30 minutes.

[0036] S12. Cultivation of seeds: the marine fungus strain Trichobotrys effusa SYSU-MS4729 was inserted into the seed culture medium, placed on a shaker at a temperature of 28° C., and cultivated at a speed of 180 rpm for 120 hours to obtain a seed culture solution.

[0037] S2. Fermentation culture:

[0038] S21. Prepare fermentation medium:

[0039] The components and dosage of the fermentation medium are: 3000 g of rice, 90 g of sea salt, 3 L of deionized water, and extinguished at 121° C. for 30 m...

Embodiment 2

[0057] Example 2 Anti-glioma Activity Test of Compound 1

[0058] The alkaloid compound (compound 1) obtained in Example 1 was subjected to a cytotoxic activity test on two glioma cell lines U251 and SNB19, and the specific steps were as follows:

[0059] 1. Experimental materials

[0060] 1.1 Tetrazole compound (CCK-8): 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene) -2H-tetrazole monosodium salt, referred to as CCK-8.

[0061] 1.2 Preparation of target cells: recovery and culture of glioma cell lines U251 and SNB19.

[0062] a. Take out the cryopreservation tubes of human glioma cell lines U251 and SNB19 from the liquid nitrogen tank, quickly place them in a 37°C water bath, shake them continuously to dissolve them quickly, and transfer them into centrifuge tubes aseptically;

[0063] b. Add DMEM complete culture solution to human glioma cell lines U251 and SNB19 respectively to 10 mL, centrifuge at 1000 rpm for 5 min, and discard the superna...

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Abstract

The invention relates to the technical field of heterocyclic compounds, in particular to an alkaloid compound derived from marine fungi, a preparation method and application thereof. The structural formula of the alkaloid compound is shown as formula (Ӏ), the alkaloid compound has anti-glioma activity, and can be applied to the preparation of anti-glioma drugs. The alkaloid compound is isolated and extracted from the fermentation product of the fungus Trichobotrys effusa SYSU-MS4729;

Description

technical field [0001] The present invention relates to the field of heterocyclic compounds, more specifically, to an alkaloid compound and its preparation method and application. Background technique [0002] Glioma originates from glial cells and is the most common primary malignant tumor of the central nervous system. It is a fatal disease that destroys brain functional areas such as thinking and movement. The incidence of glioma is about 6 cases per 100,000 people per year, and the National Cancer Society estimates that there are about 19,000 confirmed cases of glioma in the United States every year. Gliomas accounted for 35.2% to 61.0% of intracranial tumors, with an average of 49.7%. Because most gliomas grow infiltratingly and have poor boundaries with the surrounding brain tissue, treatment is very difficult. Most malignant gliomas, such as glioblastoma, die within a year of diagnosis. In recent years, the US FDA has approved two new drugs for the treatment of gli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/18C12P17/18A61P35/00C12R1/645
Inventor 陈森华刘岚沈鸿杰姜明华张盼盼
Owner SUN YAT SEN UNIV