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HPLC fingerprint establishing method for effective part group of periploca forrestii schltr

A technology of effective parts and establishment methods, applied in the field of establishment of pharmaceuticals and HPLC fingerprints, and can solve problems such as no

Active Publication Date: 2019-05-31
贵州中医药大学
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the research work of the HPLC fingerprints of the active parts of Caulis japonica

Method used

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  • HPLC fingerprint establishing method for effective part group of periploca forrestii schltr
  • HPLC fingerprint establishing method for effective part group of periploca forrestii schltr
  • HPLC fingerprint establishing method for effective part group of periploca forrestii schltr

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Embodiment 1

[0110] A method for establishing the HPLC fingerprint of the effective part group of S. japonica, comprising the following steps:

[0111] A method for establishing the HPLC fingerprint of the active parts of Caulis japonica, comprising the following steps:

[0112](1) Preparation of mixed reference substance solution: take neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and isochlorogenic acid C standard substance, and dilute to neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and isochlorogenic acid with methanol. The concentration of isochlorogenic acid C is 0.01mg·mL -1 Mixed reference substance solution;

[0113] (2) Preparation of the test solution: Accurately weigh 100.0 g of Teng Teng powder, extract three times with 70% ethanol under reflux, the amount of ethanol added is 8 times, 8 times, 6 times respectively, and the extraction time is 1.5h respectively , 1.5h, and 1.0h, the three filtrates were combined, suction filtered, concentrated t...

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Abstract

The invention discloses an HPLC fingerprint establishing method for an effective part group of periploca forrestii schltr. The method has the advantages of simplicity, convenience, stability, high precision, good reproducibility and the like. The method can comprehensively reflect the types and quantity of the chemical components contained in the effective part group of the periploca forrestii schltr, so as to integrally describe and evaluate the quality of the effective part group of the periploca forrestii schltr. Moreover, the effective part group fingerprint spectrum of the periploca forrestii schltr detected by the method is relatively more in chemical components, and moderate in height proportion of each characteristic peak. The baseline is more stable , the separation degree and peak shape are better, and the column efficiency is higher.

Description

technical field [0001] The invention relates to a method for establishing an HPLC fingerprint of a group of effective parts of Caulis japonica, belonging to the field of medicine technology. Background technique [0002] Black bone vine (Periploca forrestii Schltr.) belongs to the plant of the genus Periploca in the family Asclepiadaceae. It was recorded in the 2003 edition of "Quality Standards for Traditional Chinese and National Medicinal Materials in Guizhou Province". The function of eliminating numbness and relieving pain can relax tendons and activate collaterals, dispel wind and dampness; it can mainly treat rheumatoid arthritis, bruises, stomach pain, indigestion, amenorrhea, dysentery, etc. Heiguteng is one of the commonly used Miao medicines, and it is an important component of Heiguteng Zhuifeng Huoluo Capsules, Heiguteng Shenjin Tougu Liquid, and Heiguteng Shenjin Tougu Spray. [0003] Traditional Chinese medicine fingerprint is a comprehensive and quantifiable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
Inventor 刘育辰刘刚张玲玲杨洪秀杨婉珠金文渊
Owner 贵州中医药大学
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