Method for promoting endothelialization of biological heart valves

A heart valve and endothelialization technology, applied in the field of biomedical materials and medical devices, can solve the problems of limited VEGF content and stability, and achieve the effects of improving endothelialization, prolonging service life, and reducing calcification

Active Publication Date: 2019-06-04
JILIN VENUS HAOYUE MEDICAL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content and stability of VEGF based on electrostatic loading is limited

Method used

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  • Method for promoting endothelialization of biological heart valves
  • Method for promoting endothelialization of biological heart valves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The freshly collected porcine pericardium was washed with distilled water for 2 hours at 4°C and 100 RPM with shaking. Then soak in 0.625% glutaraldehyde for 24 hours. The pericardium was then soaked in 5% hyaluronic acid and 0.5 μg / ml VEGF for 24 hours. Finally, the pericardium was soaked in 10% 1,4-butanediol diglycidyl ether aqueous solution for 24 hours. Wash with distilled water.

[0021] Such as figure 1 As shown, the figure shows the preparation of hyaluronic acid through the cross-linking reaction between the hydroxyl group of hyaluronic acid and the epoxy group of hyaluronic acid hydrogel 1,4-butanediol diglycidyl ether in the preparation process of the present invention Schematic diagram of the hydrogel.

Embodiment 2

[0023] The freshly collected porcine pericardium was washed with distilled water for 2 hours at 4°C and 100 RPM with shaking. Then soak in 0.625% glutaraldehyde for 24 hours. The pericardium was then soaked in 5% hyaluronic acid and 1 μg / ml VEGF for 24 hours. Finally, the pericardium was soaked in 10% 1,4-butanediol diglycidyl ether aqueous solution for 24 hours. Wash with distilled water.

[0024] Such as figure 1 As shown, the figure shows the preparation of hyaluronic acid through the cross-linking reaction between the hydroxyl group of hyaluronic acid and the epoxy group of hyaluronic acid hydrogel 1,4-butanediol diglycidyl ether in the preparation process of the present invention Schematic diagram of the hydrogel.

experiment example

[0026] During the preparation process of Example 1 and Example 2, a control group was also set up, that is, the pericardium was soaked in 0.625% glutaraldehyde for 24 hours.

[0027] The results of the survival rate of endothelial cells are shown in Table 1, which shows that the survival rate of endothelial cells in the biofilms prepared in Example 1 and Example 2 is higher than that in the glutaraldehyde control group.

[0028]

endothelial cell viability

Glutaraldehyde control group

27.53±6.62

Example 1

58.04±8.92

Example 2

86.41±12.41

[0029] Table 1

[0030] The results of the rat subcutaneous calcification experiment using the preferred embodiment 2 to prepare the biofilm are shown in Table 2, which shows that the amount of calcium attached to the biofilm prepared in Example 2 is reduced compared with the glutaraldehyde control group.

[0031]

Linked calcium content μg / mg

Glutaraldehyde control group

...

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Abstract

The present invention discloses a method for promoting endothelialization of biological heart valves. The method comprises the following steps: glutaraldehyde cross-linked pericardium is soaked in anaqueous solution of hyaluronic acid and vascular endothelial growth factor (VEGF), and 1,4-butanediol diglycidyl ether is added to achieve in situ cross-linking of the hyaluronic acid on the pericardium. The provided method can enhance endothelialization of biological materials, reduce calcification, and potentially prolong service life.

Description

technical field [0001] The invention relates to the technical field of biomedical materials and medical devices, in particular to a method for promoting endothelialization of a biological heart valve. Background technique [0002] Bioprostheses currently used clinically are usually prepared from pig or bovine pericardium cross-linked with glutaraldehyde. However, glutaraldehyde-crosslinked biological valves still have the problem of calcification. One of the important reasons for the calcification of biological valves is the failure of endothelialization. On the body's normally functioning heart valves, endothelial cells prevent blood clotting and reduce calcification. Because glutaraldehyde has great cytotoxicity, glutaraldehyde-crosslinked biological valves are not conducive to the growth and proliferation of endothelial cells, resulting in their inability to endothelialize. [0003] Endothelial growth factor (VEGF) can promote the adhesion and proliferation of endothel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/54A61L27/22
Inventor 雷洋王云兵兰小蓉
Owner JILIN VENUS HAOYUE MEDICAL LTD
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