Preparation method for cephalopod transparent specimens

A production method and technology of cephalopods, which are applied in the field of making transparent cephalopod specimens, can solve problems such as poor preparation of cephalopod transparent specimens, achieve beautiful specimens, and avoid dehydration and shrinkage

Inactive Publication Date: 2019-06-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for making transparent cephalopod specimens, which pays more attention to corroding and transparent the surface muscles to highlight the internal struct...

Method used

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  • Preparation method for cephalopod transparent specimens
  • Preparation method for cephalopod transparent specimens
  • Preparation method for cephalopod transparent specimens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the making of squid transparent specimen

[0049] 1. Take a fresh, complete and undamaged squid specimen (length 5-13cm). In order to prevent the ink sac from breaking and contaminating the inner structure of the squid mantle, put the squid in clear water, and use a fine needle to puncture a small hole on the ink sac , Squeeze the ink sac to squeeze out the ink. When the ink sac is not black and no ink is squeezed out from the outside, it can be considered that the ink is completely discharged.

[0050] 2. Put the squid after removing the ink into 95% alcohol for 2 days to make the specimen basically dehydrated

[0051]3. Put the specimen into 2% KOH solution to make it transparent. Soak for 1-2 days to basically dissolve the pigment on the surface of the specimen and make the specimen soft

[0052] 4. Put the specimen in the dyeing agent and soak for 2 days until the specimen is completely dyed.

[0053] The preparation methods of different dyes are as...

Embodiment 2

[0064] Embodiment 2: the making of squid transparent specimen

[0065] 1. Get a fresh squid specimen (5-15cm in length) with a complete and undamaged appearance, remove the ink sac, wash the ink, put it into 95% alcohol and fix it for 2 days, so that the specimen is basically dehydrated. After fixation, it can be observed that the alcohol solution turns yellow, a large amount of oil dissolves, and flocculent precipitation can be observed at the bottom of the solution

[0066] 2. Put the specimen into 2% KOH solution to corrode and make it transparent. Soak for 1 day, so that most of the pigment on the surface of the specimen is washed away, and at the same time, the sea shell structure of the squid can be clearly seen;

[0067] 3. Put the specimen into the dyeing agent and soak for 2 days to make the specimen completely dyed.

[0068] The preparation methods of different dyes are as follows:

[0069] Alcian blue solution: Alcian blue 1.0g, 95% alcohol solution 500ml, glacia...

Embodiment 3

[0079] Embodiment 3: the making of octopus transparent specimen

[0080] 1. Take a fresh, complete and undamaged octopus specimen (length 10-21cm) to remove the ink sac, wash the ink, put it in 95% alcohol and fix it for 2 days, so that the specimen is basically dehydrated. After fixation, it can be observed that the alcohol solution turns yellow, a large amount of oil dissolves, and a flocculent precipitate is observed at the bottom of the solution

[0081] 2. Put the specimen into 2% KOH solution to corrode and make it transparent. Soak for 3 days to remove most of the pigment on the surface of the specimen

[0082] 3. Put the specimen in Alcian blue stain and soak for 2 days to make the specimen completely stained.

[0083] The preparation methods of different dyes are as follows:

[0084] Alcian blue solution: Alcian blue 1.0g, 95% alcohol solution 500ml, glacial acetic acid 100ml, distilled water 400ml

[0085] Toluidine blue solution: 1.0g toluidine blue, 500ml 95% a...

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Abstract

The invention aims to provide a preparation method for cephalopod transparent specimens. The method pays more attention to corrosion transparent treatment of surface muscles to highlight the internalstructure of the shellfish specimens, dyeing is performed by using different dyeing agents, so that the specimens are more ornamental; therefore, the problem of poor preparation effects of cephalopodtransparent specimens in the prior art is solved. Compared with a traditional method using alcohol to preserve specimens, the method provided by the invention avoids problems such as specimen dehydration shrinkage, protein denaturation and specimen rigidity; compared with a method using formalin to preserve specimens, the method using glycerol to preserve the specimens is non-toxic and safer; anddue to the effects of dyeing and corrosion transparent treatment, the specimens are more beautiful, and the prepared specimens can be used not only for scientific observation but also for art craft display.

Description

technical field [0001] The invention belongs to the technical field of animal specimen preparation, in particular to a method for preparing a cephalopod transparent specimen. Background technique [0002] At present, formalin or alcohol are mainly used to preserve aquatic biological specimens. Both solutions can preserve specimens for a long time, but both have obvious disadvantages. Specimens soaked in formalin are easy to smell, corrosive, and difficult to extract DNA; although specimens soaked in alcohol are easy to extract DNA, they tend to make the specimen stiff and difficult to observe and explore its shape. In recent years, the method of transparent fish specimens is emerging, and the technology is becoming more and more mature. This technology has the advantages of long storage time, high ornamental value, easy observation, DNA extraction, and not easy to stink. However, when this technique is applied to the field of shellfish, the effect of preparing transparent ...

Claims

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Application Information

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IPC IPC(8): A01N1/00
Inventor 陈栋林郑小东
Owner OCEAN UNIV OF CHINA
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