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Application of hedera saponin in preparation of anti-inflammatory injury medicine of vascular endothelial cells

A technology of hederaside and acute lung injury, which is applied in the application field of disaccharide chain hederaside in the preparation of anti-vascular endothelial cell inflammatory injury drugs, and can solve the protective effect of vascular endothelial cell inflammatory injury. No reports have been reported, etc. question

Active Publication Date: 2021-07-13
GUIZHOU NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protective effect of these compounds on inflammatory injury of vascular endothelial cells has not been reported

Method used

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  • Application of hedera saponin in preparation of anti-inflammatory injury medicine of vascular endothelial cells
  • Application of hedera saponin in preparation of anti-inflammatory injury medicine of vascular endothelial cells
  • Application of hedera saponin in preparation of anti-inflammatory injury medicine of vascular endothelial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Preparation of double sugar chain hedera saponin

[0076] 10 kg of dried roots of Dipsacus chuanxiong, crushed, extracted with 95% ethanol (25L×2), combined two extracts, concentrated under reduced pressure to obtain extract. The extract was dispersed in 5L of water, filtered, and the filtrate was pretreated on the AB-8 macroporous adsorption resin column, firstly eluted with water until the color of the eluent was lighter, then eluted with 80% ethanol, collected 80% ethanol to wash Deliquification, concentration under reduced pressure to obtain the total saponins of Dipsacus chuanxiong. The total saponins of Dipsacus sativa were applied to a normal-phase silica gel chromatography column, and the gradient elution of chloroform-methanol-water system was used to obtain pure compound 1. After ESI-MS and 13 C-NMR data compared with literature data, identified as Dipsacus saponin VI (Asperosaponin VI).

[0077] Asperosaponin VI: white amorphous powder, ESI-MS: m / z927.3 ([...

Embodiment 2

[0079] Preparation of double sugar chain hedera saponin

[0080] 5 kg of dried flower buds of Lonicerae pilosula, crushed, extracted with 95% ethanol (25L×2), combined two extracts, concentrated under reduced pressure to obtain extract. The extract was dispersed in 5L of water, filtered, and the filtrate was pretreated on the AB-8 macroporous adsorption resin column, and firstly eluted with water until the color of the eluent was lighter, and then eluted with 50% methanol for 2 column volumes, Then elute with 80% ethanol, collect the 80% ethanol eluate, and concentrate under reduced pressure to obtain the total saponins of C. argentina. Take 100 g of total saponins of Lonicera tomentosa, put it on a normal-phase silica gel chromatography column, and elute with a gradient of chloroform-methanol-water system to obtain pure compounds 1 and 2, which are analyzed by ESI-MS and 13 The C-NMR data were compared with the data in the literature, and they were identified as Lonicera cin...

Embodiment 3

[0084] Preparation of double sugar chain hedera saponin

[0085] 5 kg of dried flower buds of Lonicera chrysanthemum, crushed, extracted with 95% ethanol (25L×2), combined two extracts, concentrated under reduced pressure to obtain extract. The extract was dispersed in 5L of water, filtered, the filtrate was pretreated on the AB-8 macroporous adsorption resin column, and firstly eluted with water until the color of the eluent was lighter, and then eluted with 40% methanol for 2 column volumes, Then elute with 80% ethanol, collect the 80% ethanol eluate, and concentrate under reduced pressure to obtain total saponins of flavonoids (TSF). Take 100 g of total saponins of Lonicera tomentosa, put it on a normal-phase silica gel chromatography column, and elute with the gradient of chloroform-methanol-water system to obtain pure compound 1-3, which is analyzed by ESI-MS and 13 Comparing C-NMR data with literature data, they were identified as Dipsacoside B, Decaisoside E and Fulvot...

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Abstract

The invention discloses the application of a class of double sugar chain hedera saponin compounds in the preparation of drugs for resisting inflammatory injury of vascular endothelial cells. This kind of compound alleviates the inflammatory injury of endothelial cells by directly inhibiting the adsorption of inflammatory factors and adhesion molecules, regulating NF-κB signaling pathway and other ways, and plays a protective role in the inflammatory injury of vascular endothelial cells. The application of this type of double sugar chain hedera saponins in the preparation of drugs against inflammatory injury of vascular endothelial cells is disclosed for the first time, and the sources of raw materials are abundant and the preparation methods are mature. It has good value in the development of drugs for the treatment of toxicity, atherosclerosis and diabetic vascular complications.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of a class of double sugar chain hedera saponins in the preparation of anti-vascular endothelial cell inflammatory injury drugs. Background technique [0002] Vascular endothelial cells (VEC) are a selectively permeable barrier covering between the blood vessel wall and the blood flow. They can synthesize and secrete substances that regulate the coagulation-fibrinolytic system, factors that regulate vascular tension, and cytokines. It plays an important role in angiogenesis, wound healing and inflammatory response (Endemann DH, et al. Endothelial dysfunction. JAm Soc Nephrol. 2004, 15(8): 1983–92.), and is an important part of the first barrier against foreign invasion part. When it senses external stimuli, it synthesizes and releases a variety of factors to regulate and exert functions such as hemostasis, cell growth, vasoconstr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61P11/00A61P13/12A61P31/04A61P3/10A61P9/10A61P9/14A61P29/00A61P17/02
Inventor 杨庆雄孙黔云郭靖刘巧洲张军
Owner GUIZHOU NORMAL UNIVERSITY
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