Preparation method of mesenchymal stem cell artificial pancreas islet
A technology of stem cells and pancreatic islets, applied in the field of biomedicine, can solve the problems of accelerating the vascularization of artificial pancreatic islets, the large number of pancreatic islets, and strong immune rejection.
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Embodiment 1
[0120] A preparation method of mesenchymal stem cell artificial islets, comprising the following steps:
[0121] (1) Mesenchymal stem cells are cultured and induced to generate islet-like cells;
[0122] (2) Extraction and cultivation of vascular endothelial cells;
[0123] (3) Preparation of thermosensitive chitosan hydrogel;
[0124] (4) preparing composite hydrogel;
[0125] (5) After local transplantation of the composite hydrogel obtained in step (4), agglutination occurs rapidly in a body temperature environment of 37° C. to form artificial islets.
[0126] Further, the mesenchymal stem cells in step (1) are umbilical cord mesenchymal stem cells, which include:
[0127] (11) Extraction of umbilical cord mesenchymal stem cells
[0128] (111) Under aseptic conditions, take a healthy umbilical cord specimen of 10 cm and place it in 1% PBS buffer containing double antibodies;
[0129] (112) In the ultra-clean workbench, repeatedly rinse the umbilical cord specimen with PB...
Embodiment 2
[0167] A preparation method of mesenchymal stem cell artificial islets, comprising the following steps:
[0168] (1) Mesenchymal stem cells are cultured and induced to generate islet-like cells;
[0169] (2) Extraction and cultivation of vascular endothelial cells;
[0170] (3) Preparation of thermosensitive chitosan hydrogel;
[0171] (4) preparing composite hydrogel;
[0172] (5) After local transplantation of the composite hydrogel obtained in step (4), agglutination occurs rapidly in a body temperature environment of 37° C. to form artificial islets.
[0173] Further, the mesenchymal stem cells in step (1) are umbilical cord mesenchymal stem cells, which include:
[0174] (11) Extraction of umbilical cord mesenchymal stem cells
[0175] (111) Under aseptic conditions, take a healthy umbilical cord specimen of 20 cm and place it in 1% PBS buffer containing double antibodies;
[0176] (112) In the ultra-clean workbench, repeatedly rinse the umbilical cord specimen with ...
Embodiment 3
[0213] A preparation method of mesenchymal stem cell artificial islets, comprising the following steps:
[0214] (1) Mesenchymal stem cells are cultured and induced to generate islet-like cells;
[0215] (2) Extraction and cultivation of vascular endothelial cells;
[0216] (3) Preparation of thermosensitive chitosan hydrogel;
[0217] (4) preparing composite hydrogel;
[0218] (5) After local transplantation of the composite hydrogel obtained in step (4), agglutination occurs rapidly in a body temperature environment of 37° C. to form artificial islets.
[0219] Further, the mesenchymal stem cells in step (1) are umbilical cord mesenchymal stem cells, which include:
[0220] (11) Extraction of umbilical cord mesenchymal stem cells
[0221] (111) Under sterile conditions, take a healthy umbilical cord specimen of 15 cm and place it in 1% PBS buffer containing double antibodies;
[0222] (112) In the ultra-clean workbench, repeatedly rinse the umbilical cord specimen with ...
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