Unlock instant, AI-driven research and patent intelligence for your innovation.

Application and kit of hsa-mir-15b-5p as a molecular marker for acute altitude sickness susceptible persons

A technology of hsa-mir-15b-5p and molecular markers, applied in the field of molecular biotechnology and its application, to achieve the effect of reducing health and life threats, strong reliability, reliable prevention and treatment

Active Publication Date: 2022-03-29
ARMY MEDICAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the correlation between circulating microRNAs and AMS susceptibility.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application and kit of hsa-mir-15b-5p as a molecular marker for acute altitude sickness susceptible persons
  • Application and kit of hsa-mir-15b-5p as a molecular marker for acute altitude sickness susceptible persons
  • Application and kit of hsa-mir-15b-5p as a molecular marker for acute altitude sickness susceptible persons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Correlation between the expression of microRNA in plasma samples and the risk of AMS

[0043] 1. Material and Specimen Collection Description

[0044] Plasma samples of AMS-susceptible individuals were collected from 209 AMS patients who entered the plateau rapidly before entering the plateau. Plasma samples of normal people were collected from 203 normal healthy people who entered the plateau rapidly before entering the plateau. The diagnosis of AMS was confirmed by the International Common Diagnostic Criteria-Lake Lewis Score. All the population did not take any preventive medicine before blood sampling. A total of 2ml of blood was collected in EDTA-Na anticoagulant tubes for each sample. The clinical case characteristics of the patients are shown in Table 1. This study has been approved by the Medical Ethics Committee of the Army Military Medical University (Third Military Medical University), and all specimens were collected with the informed consent o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a microRNA marker for screening acute altitude sickness susceptible persons, and the microRNA is hsa-miR-15b-5p as a molecular marker for acute altitude sickness susceptible persons, which can be used in the preparation and screening of acute altitude sickness Applied in reagents or kits for susceptible persons. It also provides a kit for detecting the hsa-miR-15b-5p molecule in human plain plasma by real-time fluorescent quantitative PCR method, and predicts the risk of acute altitude sickness by its expression level. As a molecular marker, the microRNA has the characteristics of strong specificity, high sensitivity, non-invasiveness, and convenience, and is suitable for large-scale screening of people susceptible to acute altitude sickness in plains.

Description

technical field [0001] The invention belongs to the field of molecular biotechnology and its application. The invention relates to the application and kit of hsa-miR-15b-5p as a molecular marker for acute altitude sickness susceptible persons. Check the risk of acute altitude sickness. Therefore, it can be used to assess the susceptibility of the human body to acute altitude sickness and reduce the risk of acute altitude sickness. Background technique [0002] Acute mountain sickness (AMS) occurs in plain people who have lived in low-altitude areas for a long time, 1-3 days after entering the plateau quickly without adapting to the new environment, including headache, dizziness, fatigue, insomnia , Emotional restlessness, flatulence, diarrhea and vomiting and a series of symptoms, among which severe headache is a typical symptom of AMS. In geography, a vast area with an altitude higher than 500m and relatively flat or undulating terrain is called a plateau. In medicine, a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
Inventor 高钰琪徐刚黄河鄂国基孙滨达李鹏柯贤峰董华平陈建张二龙
Owner ARMY MEDICAL UNIV