COX-2 activity inhibition-based traditional Chinese medicine composition detection method
A COX-2 and detection method technology, applied in the field of medicine, can solve problems such as not reflecting the overall drug effect, and achieve high precision and good repeatability
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Embodiment 1
[0130] Prescription: 255g forsythia, 255g honeysuckle, 85g roasted ephedra, 85g fried bitter almond, 255g gypsum, 255g isatis root, 255g Mianma Guanzhong, 255g houttuynia cordata, 85g patchouli, 51g rhubarb, 85g rhodiola, mint Brain 7.5g, licorice 85g;
[0131]Preparation method: the above thirteen flavors, patchouli plus water distillation to extract the volatile oil, collect the volatile oil, filter the water extract, and set aside; forsythia, ephedra, houttuynia, rhubarb are extracted twice with 70% ethanol, the first time 2 1.5 hours for the second time, filter the extract, combine, recover ethanol, and set aside; add honeysuckle, gypsum, isatis root, Mianma Guanzhong, licorice, and rhodiola to boiling, add fried bitter almonds, and cook for two 1.5 hours for the first time, 1 hour for the second time, filter the decoction, combine the filtrates, add the aqueous solution after patchouli oil extraction, concentrate to a relative density of 1.10-1.15 (60°C), add ethanol to m...
Embodiment 2
[0137] Bioassay method:
[0138] Preparation of the reference substance: Accurately weigh the working reference substance of the traditional Chinese medicine composition (the standardized potency of pure rofecoxib chemical product is 1000U μg -1 ), grind finely, add methanol solution for ultrasonic extraction for 30min, centrifuge at 10000r / min for 10min, take the supernatant to make each 1ml contain 1.2×10 5 U solution, that is.
[0139] Preparation of the test product: Take an appropriate amount of the content of the traditional Chinese medicine composition, grind it finely, add DMSO for ultrasonic extraction for 30 minutes, centrifuge at 10000r / min for 10 minutes, take the supernatant, and make it according to the estimated potency before use. Each 1ml contains 1.2×10 5 U solution, that is.
[0140] Determination method: Take the reference substance solution and the test solution at a dilution ratio of 1:0.8 to 1:0.5, operate according to the instructions of the commercia...
Embodiment 3
[0142] Bioassay method:
[0143] Preparation of the reference substance: Accurately weigh the working reference substance of the traditional Chinese medicine composition (the standardized potency of pure celecoxib chemical product is 1000U μg -1 ), grind finely, add ethanol solution and ultrasonically extract for 30min, centrifuge at 10000r / min for 10min, take the supernatant and make each 1ml contain 1.2×10 5 U solution, that is.
[0144] Preparation of the test product: Take an appropriate amount of the content of the traditional Chinese medicine composition, grind it finely, add DMSO for ultrasonic extraction for 30 minutes, centrifuge at 10000r / min for 10 minutes, take the supernatant, and make it according to the estimated potency before use. Each 1ml contains 1.2×10 5 U solution, that is.
[0145] Determination method: Take the reference substance solution and the test solution according to the dilution ratio between 1:0.8 and 1:0.5, and measure the inhibition rate acc...
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