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A kind of collagen tripeptide and its production method and application

A collagen and production method technology, applied in the field of collagen tripeptide, can solve the problems of low enzymatic hydrolysis efficiency, complicated operation, acid and alkali resistance, etc., and achieve low free amino acid content, simplified process steps, and not easy to absorb moisture and agglomerate Effect

Active Publication Date: 2020-06-26
卓康(福建)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the problems and shortcomings of the above two invention patent applications are: the enzymatic hydrolysis efficiency of the enzymes used is low, the enzymatic hydrolysis time is long, the amount of enzyme added is large, and the optimum pH value and optimum temperature need to be adjusted for enzymolysis. Step enzymatic hydrolysis, complex operation, adjusting the optimum pH value of enzymatic hydrolysis will bring in a large amount of inorganic ions, and controlling the optimum enzymatic hydrolysis temperature consumes a lot of energy; ultrafiltration membrane filtration is required, and the role of ultrafiltration membrane is to intercept large Molecular substances, the membrane core used is an organic material, not resistant to acid and alkali, high temperature and high pressure, small flux, short service life, and inconvenient to clean; the prepared collagen tripeptide powder has poor water resolubility, and during storage easy to absorb moisture

Method used

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  • A kind of collagen tripeptide and its production method and application
  • A kind of collagen tripeptide and its production method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Raw material pre-treatment: put marine fish (large yellow croaker) scales into the reaction kettle, add an alkali solution (sodium hydroxide solution, the same below) twice the weight of fish scales, stir and wash for 12 hours, to remove the fat and Wash other impurities with water, then add an acid solution (hydrochloric acid solution, the same below) twice the weight of fish scales, stir and wash for 12 hours, then wash with deionized water, add water 3 times the weight of fish scales and grind to prepare a crude collagen solution. Wherein, the mass volume concentration of the alkali solution is 4%, and the mass volume concentration of the acid solution is 3%.

[0046] (2) Heat treatment: heat the crude collagen solution in hot water at 100° C. for 30 minutes, and then lower the temperature to 50-55° C. to obtain a denatured collagen solution.

[0047] (3) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the denatur...

Embodiment 2

[0053] (1) Raw material pre-treatment: put freshwater fish (grass carp) scales into the reaction kettle, add an alkali solution 10 times the weight of the scales, stir and wash for 1 hour to remove the fat and other impurities of the scales, wash them with water, and then add the scales 10 times the weight of the acid solution, stirred and washed for 1 hour, then washed with deionized water, added with water 4 times the weight of fish scales and ground to prepare a crude collagen solution. Wherein, the mass volume concentration of the alkali solution is 1%, and the mass volume concentration of the acid solution is 8%.

[0054] (2) Heat treatment: heat the crude collagen solution in hot water at 100°C for 30 minutes, then cool down to 50-55°C to obtain a denatured collagen solution;

[0055] (3) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the denatured collagen solution, mix evenly, and enzymolyze for 1 hour to prepare a col...

Embodiment 3

[0061] (1) Raw material pre-treatment: Put freshwater fish (crucian carp) fish scales into the reaction kettle, add an alkali solution 6 times the weight of the fish scales, stir and wash for 6 hours to remove the fat and other impurities of the fish scales, wash them with water, and then add the fish scales Acid solution 6 times the weight was stirred and washed for 6 hours, then washed with deionized water, added with water 5 times the weight of fish scales and ground to prepare a crude collagen solution. Wherein, the mass volume concentration of the alkali solution is 2%, and the mass volume concentration of the acid solution is 5%.

[0062] (2) Heat treatment: heat the crude collagen solution in hot water at 100°C for 30 minutes, then cool down to 50-55°C to obtain a denatured collagen solution;

[0063] (3) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the denatured collagen solution, mix evenly, and enzymolyze for 1 hou...

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Abstract

The invention belongs to the field of collagen tripeptide, and discloses collagen tripeptide as well as a production method and an application thereof. The production method of the collagen tripeptidecomprises the following steps: alkaline washing, acid washing and grinding with water on fish scales, heating treatment, enzymolysis of a high-efficiency compound enzyme preparation under the conditions that pH is not adjusted and the temperature is not controlled, removal of components which are not prone to dissolution and moisture absorption through filtration by an inorganic nanofiltration ceramic membrane, high-temperature and high-pressure treatment, membrane concentration and fluidized bed drying for preparation of powder. By adopting the method, enzymolysis pH and enzymolysis temperature are not required to be adjusted, enzymolysis time is short, the enzyme addition amount is small, enzymolysis products have low molecular weight and are concentrated, and the prepared collagen tripeptide powder has excellent water solubility and is not prone to moisture absorption in the storage process; and common food, health food, infant formula food, formula food for special medical purposes and beauty and skin care products can be prepared from the collagen tripeptide powder.

Description

technical field [0001] The invention belongs to the field of collagen tripeptides, in particular to a collagen tripeptide and its production method and application. Background technique [0002] Collagen is rich in amino acids such as glycine, proline, and hydroxyproline that the human body needs. Collagen is a high-molecular functional protein. It is the main component of the skin, accounting for 80% of the skin's dermis. It forms a fine elastic network in the skin, which firmly locks moisture and supports the skin. Collagen is a helical fibrous protein twisted by three peptide chains. It is also the most abundant protein in the human body. It is widely distributed in connective tissue, skin, bone, interstitial cells of internal organs, muscle cavity, ligament, sclera and other parts. Accounting for more than 30% of the total protein in the human body, it is rich in proline, hydroxyproline and other collagen characteristic amino acids needed by the human body, and is an im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34A23L33/18A23L29/00A61K8/65A61Q19/00
Inventor 郑志镪
Owner 卓康(福建)生物科技有限公司
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