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Application of human beta-defensin 1 in promoting killing activity of NK (natural killer) cells

A technology of human defensins and NK cells, applied in the field of biomedicine, can solve the problem that the immunomodulatory effects of defensins are poorly understood, and achieve the effects of low cost, wide antibacterial spectrum and high safety.

Inactive Publication Date: 2019-06-21
上海众择生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is known about the immunomodulatory role of defensins

Method used

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  • Application of human beta-defensin 1 in promoting killing activity of NK (natural killer) cells
  • Application of human beta-defensin 1 in promoting killing activity of NK (natural killer) cells
  • Application of human beta-defensin 1 in promoting killing activity of NK (natural killer) cells

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Separation and purification of human peripheral blood NK cells:

[0022] 1) Take 1ml of human peripheral intravenous heparin anticoagulant blood, add 1ml PBS to half-dilute;

[0023] 2) Add 1ml of lymphocyte separation solution to a 15ml centrifuge tube;

[0024] 3) Use a capillary pipette to slowly add the diluted blood into the separation solution test tube along the tube wall, so that an interface with the separation solution is formed, and the two cannot be mixed;

[0025] 4) At room temperature, 2200rpm, increase speed 5, decrease speed 0, centrifuge for 23 minutes;

[0026] 5) Carefully absorb the mononuclear cell (PBMC) layer with a capillary pipette, place it in a new 15ml centrifuge tube, add more than 5 times of PBS, wash at 1500rpm for 10 minutes, and wash twice to obtain human peripheral blood PBMC;

[0027] 6) Resuspend the PBMC obtained above in RoboSep TM In the buffer, adjust the cell concentration to 5×10 7 / ml;

[0028] 7) Add EasySep accordin...

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Abstract

The invention discloses a novel use of a human defensin protein in regulating killing activity of NK (natural killer) cells. The human defensin protein is HBD1 (human beta-defensin 1), which is a polypeptide to the human natural immune system and has an amino acid sequence shown as SEQ ID NO. 1. The HBD1 herein can effectively promote NK cells' killing activity against cancer cells, and has potential pharmaceutical use.

Description

technical field [0001] The invention relates to the application of human beta-defensin 1 in promoting the killing activity of NK cells, belonging to the field of biomedicine. Background technique [0002] After being stimulated by external pathogenic microorganisms, the normal body resists infection and performs immune defense functions through a series of innate and adaptive immune responses. Among them, innate immunity is the first line of defense acquired by the body innately. It appears early and responds quickly, and plays an important role in the process of eliminating pathogens by the body. NK cells (Natural Killer Cells, NK cells) are an important class of innate immune cells in the body. Because their killing effect occurs early after acting on target cells, their killing activity is not limited by MHC, and they do not depend on antibodies, so they are called natural killer cells. NK cells are not only related to anti-tumor, anti-viral infection and immune regulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17C12N5/0783A61P35/00A61P35/02
Inventor 杜飞
Owner 上海众择生物科技有限公司