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Application of Prevotella in identifying and/or distinguishing individuals of different nationalities

A technology of Prevotella and intermediate Prevotella, applied in the direction of microorganism-based methods, microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems of undisclosed application of Prevotella genus

Active Publication Date: 2019-06-21
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The prior art does not disclose the use of Prevotella to identify and / or differentiate the ethnicity of a population

Method used

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  • Application of Prevotella in identifying and/or distinguishing individuals of different nationalities
  • Application of Prevotella in identifying and/or distinguishing individuals of different nationalities
  • Application of Prevotella in identifying and/or distinguishing individuals of different nationalities

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Using the relative abundance of Prevotella in individual feces to identify a subject as Tibetan or Han

[0066] (1) Determining the abundance of said microbial marker in said individual's stool sample:

[0067] 1) Collect the feces of the individual to be tested, and extract the DNA of the microorganisms in the feces:

[0068] Collect the feces of the individual to be tested, draw 1000ul of CTAB lysate into a 2.0ml EP tube, add lysozyme, add about 500ul of the sample into the lysate, put it in a water bath at 65°C, and mix it upside down several times during this period to fully lyse the sample. Take the supernatant by centrifugation, add phenol (pH8.0): chloroform: isoamyl alcohol (25:24:1), mix by inverting, and centrifuge at 12000rpm for 10min. Take the supernatant, add chloroform:isoamyl alcohol (24:1), mix by inverting, and centrifuge at 12000rpm for 10min. Pipette the supernatant into a 1.5mL centrifuge tube, add isopropanol, shake up and down, and pre...

Embodiment 2

[0080] Example 2 Feasibility of Using Relative Abundance of Prevotella to Discriminate Ethnicity of Subjects Identification of Study Populations:

[0081] The subjects were male soldiers serving in China. The experimental group consisted of 128 Tibetan male soldiers accustomed to plateaus above 3,500 meters above sea level. The control group consisted of 128 plain Han soldiers who were age-matched with Tibetan soldiers. Strictly control the two groups of people to have the same mixed diet of Chinese troops, maintain the same training environment and training intensity at an altitude of 3500 meters, quit smoking and drinking, and last for 3 months. Soldiers with chronic inflammatory diseases, oral antibiotics, acute infections, and gastrointestinal disorders were excluded.

[0082] method:

[0083] (1) Determining the abundance of said microbial marker in said individual's stool sample:

[0084] 1) Collect the feces of the individual to be tested, and extract the DNA of the m...

Embodiment 3

[0100] Example 3 Constructing a ROC curve to compare and evaluate the ability of the relative abundance of bacteria to identify plateau Han population and Tibetan population

[0101] The receiver operating curve (ROC) method was used for verification, and the relative abundance of Prevotella in the feces of the subjects was used to judge its ability to identify the plateau Han population and the Tibetan population. Differences were compared using the Mann-Whitney test because the samples were not normally distributed. The ROC curve was drawn to evaluate the sensitivity and specificity of the relative abundance of the bacterial genera in the differential diagnosis of the plateau Han population and the Tibetan population, and the cut-off value was calculated. P<0.05 was considered statistically significant, and the SPSS22.0 software package was used for statistical processing.

[0102] The result is as figure 2 shown by figure 2 The results showed that the ROC curve showed t...

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Abstract

The invention provides application of microbial markers in identifying and / or distinguishing individuals of different nationalities. The microbial markers are of the genus Prevotella.

Description

technical field [0001] The invention belongs to the field of biological identification and / or differentiation, and specifically relates to the application of Prevotella in identifying and / or distinguishing individuals of different races. Background technique [0002] There are a large number of symbiotic flora in the human intestinal tract, the number of which exceeds 1000 trillion, which is about 10 times the total number of human cells. At the same time, the number of microbial genes in the intestinal tract is about 3 million, which is about 100 times the number of genes in the human genome. Such a large number of genes can help microorganisms adapt to the changing environment and form an inseparable symbiotic relationship with the human body. Residents with different genetic backgrounds and living habits have great differences in the composition of intestinal flora. For example, Bacteroides is the most abundant and inter-individual bacterial genus in the intestines of Wes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/689C12R1/01
Inventor 何昆仑刘继轩赵晓静高晓健贾志龙张泽宇姜辉源朱雨刘镕珲
Owner GENERAL HOSPITAL OF PLA
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