Pretreatment method of minodronic acid in plasma sample based on derivatization on solid-phase extraction column and detection method

Abstract

The invention discloses a pretreatment method of minodronic acid in a plasma sample based on derivatization on a solid-phase extraction column and a detection method, and belongs to the field of biological analysis. The detection method provided by the invention comprises the following steps: firstly, carrying out pretreatment on the plasma sample containing minodronic acid, and then detecting theconcentration of minodronic acid in the plasma by using a liquid phase-mass spectrometry method, wherein the pretreatment comprises the following steps: injecting the plasma sample containing minodronic acid into the activated solid-phase extraction column, leaching, adding a derivatization reagent, carrying out a column derivatization reaction, collecting a reaction solution, blow-drying by using nitrogen, redissolving, and directly injecting a supernatant. According to the method, the defect that minodronic acid cannot be subjected to liquid phase analysis due to the polarity of minodronicacid is overcome, compared with an existing derivatization method, the dosage of plasma is greatly reduced, and sample pretreatment steps are simplified.

Description

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AI Technical Summary

Benefits of technology

The present in this patented technology uses different types of compounds called dodecadimidocyanatoiron(DDC), tris(2-(dimethoxyphenol))dihydroxybenzoquinone-2-aceticacetamide dimerized with 2'-hydrazine sulfite salt or triazole ammonium chloroform resin). These materials help improve upon various aspects like purification efficiency during protein production processes. Different methods may include liquid chromatography techniques, gas chromatographic separation, gel eletrospray devices, etc., resulting in better results compared to previous methodologies. Overall, these technological feates result in improved quality control over biotherapeptides obtained via peptide synthesis procedures.

Problems solved by technology

The technical problem addressed in this patented research relates to developing effective methods that can accurately measure small amounts of certain types of molecules called mineral hydroxyproline (minoheptanoid).

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