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Serum-free suspension culture type MDCK cell strain and application thereof

A suspension culture, MDCK-XF03 technology, applied in the field of cell culture, can solve problems such as technological difficulties

Inactive Publication Date: 2019-06-25
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Bioreactor culture cells include microcarrier culture, sheet carrier culture, low serum suspension culture and serum-free suspension culture, etc. Among them, microcarrier culture and sheet carrier culture are adherent culture methods, and animal serum needs to be added to the medium , and there are technical difficulties when the process is scaled up. The largest single tank culture volume in China is less than 500L

Method used

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  • Serum-free suspension culture type MDCK cell strain and application thereof
  • Serum-free suspension culture type MDCK cell strain and application thereof
  • Serum-free suspension culture type MDCK cell strain and application thereof

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Embodiment

[0019] 1. Materials

[0020] 1.1 MDCK cell line (CCL-34), introduced from ATCC, the cell passage was P55 at the time of introduction, the preservation number in ATCC: 60245139, the frozen storage time in ATCC: 2011-10-20, it is an adherent cultured cell. After the introduction, according to the requirements of the 2010 edition of the Pharmacopoeia, a master cell bank (number: MDCK-M-60) was established when the culture reached the 60th generation, and the number of frozen storage was 1560; a working cell bank was established when the culture reached the 63rd generation (number: For: MDCK-W-63) The frozen storage quantity is 1680 pieces. MDCK-M-60 and MDCK-W-63 were submitted to the China National Institutes for Food and Drug Control for inspection in December 2014 and January 2015, respectively, and obtained a qualified cell inspection report in December 2017. The report numbers are SH201702634 and SH201702584.

[0021] 1.2 DEME (high-glucous) medium, Gibco, product number: ...

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Abstract

The invention discloses a serum-free suspension culture type MDCK cell strain and application thereof. The serum-free suspension culture type MDCK cell strain is preserved at Chinese Center for Type Culture Collection, and is classified and named as follows: Madin-Darby canine kidney cells MDCK-XF03 with the preservation number: CCTCC NO:C2018191; the serum-free suspension culture type MDCK cell strain can be applied to production of influenza vaccines and avian influenza vaccines. The serum-free suspension culture type MDCK-XF03 cell line is obtained through serum-free adaptive culture and suspension culture domestication, Madin-Darby canine kidney cells MDCK-XF0 suspension culture in serum-free medium has high activity, few clusters and good uniformity, is completely suitable for suspension culture in a serum-free medium, and the serum-free suspension culture type cell strain is provided for relevant research and production of influenza and avian influenza vaccines in China.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a serum-free suspension culture type MDCK cell strain and application thereof. Background technique [0002] Influenza is influenza (Influenza), which is an acute respiratory infectious disease caused by influenza virus. Bird flu (Bird Flu or Avian Influenza) is the abbreviation of avian influenza. (also claiming avian influenza virus) causes a kind of acute infectious disease, also can infect the mankind, and the death rate after the human infection bird flu is more than 33%. So far, neither influenza nor avian influenza has found an ideal drug for treatment. Vaccination is still the most effective means to prevent the occurrence and prevalence of diseases today. [0003] The traditional production process of influenza vaccines and avian influenza vaccines is the chicken embryo culture method. This method of using chicken embryos as the production substrate has...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N7/00
Inventor 马忠仁乔自林靳冬武刘振斌令世鑫柏家林王家敏
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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