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Macadimia nut protein peptide with antifatigue effect and preparation method and application thereof

A nut protein and anti-fatigue technology, applied in the field of macadamia nut protein peptide and its preparation, can solve the problems of less amino acid types, low extraction rate, low protein peptide activity, etc., and achieve the effect of delaying physical fatigue

Inactive Publication Date: 2019-07-09
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation methods of macadamia nut protein peptides are generally obtained by enzymatic hydrolysis, ultrasonic-assisted digestion or alcohol extraction, but there are problems such as low activity of the protein peptide, few types of amino acids contained, and low extraction rate. In addition, using The cost of recovering protein peptides by ultrafiltration and other methods is relatively high

Method used

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  • Macadimia nut protein peptide with antifatigue effect and preparation method and application thereof
  • Macadimia nut protein peptide with antifatigue effect and preparation method and application thereof
  • Macadimia nut protein peptide with antifatigue effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of the macadamia nut protein peptide with anti-fatigue effect in this example is as follows. First, the shelled macadamia nuts are crushed at low temperature, and then the crushed macadamia nuts are added with Tris-HCl buffer and neutral protease for ultrasonic assistance. Dissolve, wherein the concentration of Tris-HCl buffer is 0.5M, wherein the pH of Tris-HCl buffer is 6.8, after centrifugation, take the supernatant and use macroporous adsorption resin for protein peptide adsorption, and the macroporous adsorption resin is eluted multiple times Collect protein peptides and freeze-dry to obtain macadamia nut protein peptide powder;

[0026] Wherein, the crushed particle size of the macadamia nuts is 300 μm;

[0027] Wherein, the dissolution temperature of the ultrasonic-assisted dissolution is 30°C, the ultrasonic power is 200W, and the ultrasonic time is 40min;

[0028] Wherein, the skeleton of the macroporous adsorption resin is selected from...

Embodiment 2

[0031] The preparation method of the macadamia nut protein peptide with anti-fatigue effect in this example is as follows. First, the shelled macadamia nuts are crushed at low temperature, and then the crushed macadamia nuts are added with Tris-HCl buffer and neutral protease for ultrasonic assistance. Dissolve, the concentration of the Tris-HCl buffer is 0.5M, the pH of the Tris-HCl buffer is 7.0, after centrifugation, take the supernatant and use the macroporous adsorption resin for protein peptide adsorption, and the macroporous adsorption resin is eluted multiple times Collect protein peptides and freeze-dry to obtain macadamia nut protein peptide powder;

[0032] Wherein, the crushed particle size of the macadamia nuts is 200 μm;

[0033] Wherein, the dissolution temperature of the ultrasonic-assisted dissolution is 35°C, the ultrasonic power is 200W, and the ultrasonic time is 30min;

[0034] Wherein, the skeleton of the macroporous adsorption resin is selected from acr...

Embodiment 3

[0037] The preparation method of the macadamia nut protein peptide with anti-fatigue effect in this example is as follows. First, the shelled macadamia nuts are crushed at low temperature, and then the crushed macadamia nuts are added with Tris-HCl buffer and neutral protease for ultrasonic assistance. Dissolve, wherein the concentration of the Tris-HCl buffer is 0.5M, wherein the pH of the Tris-HCl buffer is 6.9, after centrifugation, take the supernatant and use the macroporous adsorption resin for protein peptide adsorption, and the macroporous adsorption resin is eluted multiple times Collect protein peptides and freeze-dry to obtain macadamia nut protein peptide powder;

[0038] Wherein, the crushed particle size of the macadamia nuts is 250 μm;

[0039] Wherein, the dissolution temperature of the ultrasonic-assisted dissolution is 33°C, the ultrasonic power is 200W, and the ultrasonic time is 35min;

[0040] Wherein, the skeleton of the macroporous adsorption resin is s...

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Abstract

The invention discloses macadimia nut protein peptide with an antifatigue effect and a preparation method and application thereof, and belongs to the technical field of food engineering. The preparation method of the macadimia nut protein peptide with the antifatigue effect comprises the steps that firstly, hulled macadimia nuts are subjected to low-temperature smashing; secondly, Tris-HCl buffering liquid and neutral protease are added to the smashed macadimia nut for ultrasonic auxiliary dissolving, wherein the concentration of the Tris-HCl buffering liquid is 0.5 M, and the pH of the Tris-HCl buffering liquid ranges from 6.8 to 7.0; thirdly, after centrifugation is carried out, supernate is taken and subjected to protein peptide adsorption with macroreticular resin, the macroreticular resin is eluted many times, the protein peptide is collected, freeze drying is carried out, and macadimia nut protein peptide powder is obtained. A solid drink comprises the macadimia nut protein peptide, soybean meal, polyglycerol-6 distearate, taurine, hawthorn fruit powder, pectin, sodium carboxymethylcellulose, sodium chloride and potassium chloride. The prepared solid drink containing the macadimia nut protein peptide has the remarkable antifatigue effect.

Description

technical field [0001] The invention belongs to the technical field of food engineering, and in particular relates to a macadamia nut protein peptide with anti-fatigue effect and its preparation method and application. Background technique [0002] Macadamia ternifolia F.Muell., also known as Australian walnut, macadamia nut, etc., belongs to the genus of macadamia in the family Longanaceae. The edible part of the macadamia nut is the kernel, which is rich in nutritional value and contains 9% protein and 78% oil. The protein contains 8 kinds of amino acids necessary for the human body, and the protein peptide prepared from its protein has a variety of biological activities. Protein peptide is a general term for different peptides composed of amino acids in different compositions and arrangements, ranging from dipeptides to complex linear and circular structures. It is a multifunctional compound derived from proteins, and many protein peptides also have original proteins or t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23J3/14A23L2/39A23L2/52A23L2/66A23L33/10A23L33/18
CPCA23J1/148A23J1/146A23J1/142A23J3/14A23L33/18A23L33/10A23L2/39A23L2/66A23L2/52A23V2002/00A23V2250/21A23V2200/30
Inventor 帅希祥杜丽清马飞跃张明涂行浩
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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