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A method for treating reclaimed water containing Chryseobacterium meningosepticum

A golden bacterium and septic technology, which is applied in the field of inhibiting the infection risk of meningoseptica aureus in water, can solve the problems of population outbreak risk and substandard bacterial population suppression, and achieve the effect of reducing infection risk.

Active Publication Date: 2021-08-17
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a method for treating reclaimed water containing Chryseobacterium meningosepticum, so as to solve the risk of population outbreak of Chryseobacterium meningosepticum existing in the reclaimed water, and the problem of substandard inhibition of the flora

Method used

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  • A method for treating reclaimed water containing Chryseobacterium meningosepticum
  • A method for treating reclaimed water containing Chryseobacterium meningosepticum
  • A method for treating reclaimed water containing Chryseobacterium meningosepticum

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Effect test

Embodiment 1

[0029] Include the following steps:

[0030] (a) adding a biological flocculant solution to the regenerated water containing aureus meningoseptica, stirring evenly, the volume ratio of the regenerated water containing aureus meningosepticum to the biological flocculant solution is 90:5, and the biological flocculant solution The concentration of 3g / ml;

[0031] (b) The pNJR6 plasmid was transformed into E. coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as the lethal plasmid donor bacteria;

[0032] (c) adding 10 to the regeneration water of Aureus meningosepticum to which the biological flocculant solution was added in step (a). 3pcs / L lethal plasmid donor bacteria, and adding 50mg / L nanoscale graphene oxide, pH value 7, the lethality rate of meningoseptica aureus was greater than 50% after 24 hours, and the lethality rate after 36 hours was acceptable more than 80%;

[0033] (d) After 60 hours, add a bio...

Embodiment 2

[0035] Include the following steps:

[0036] (a) adding a biological flocculant solution to the regenerated water containing aureus meningoseptica, and stirring evenly, the volume ratio of the regenerated water containing aureus meningosepticum to the biological flocculant solution is 90:8, and the biological flocculant solution is 90:8. The concentration of 4g / ml;

[0037] (b) The pNJR6 plasmid was transformed into E. coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as the lethal plasmid donor bacteria;

[0038] (c) adding 10 to the regeneration water of Aureus meningosepticum to which the biological flocculant solution was added in step (a). 3 pcs / L lethal plasmid donor bacteria, and adding the concentration of 65mg / L nanoscale graphene oxide, the pH value is 7, the lethality rate of meningoseptica aureus is greater than 50% after 24 hours, and the lethality rate after 36 hours can be more than 80%;

[00...

Embodiment 3

[0041] Include the following steps:

[0042] (a) adding a biological flocculant solution to the regenerated water containing aureus meningoseptica, stirring evenly, the volume ratio of the regenerated water containing aureus meningosepticum to the biological flocculant solution is 90:10, and the biological flocculant solution is 90:10. The concentration of 5g / ml;

[0043] (b) The pNJR6 plasmid was transformed into E. coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as the lethal plasmid donor bacteria;

[0044] (c) adding 10 to the regeneration water of Aureus meningosepticum to which the biological flocculant solution was added in step (a). 3 pcs / L lethal plasmid donor bacteria, and adding the concentration of 80mg / L nanoscale graphene oxide, the pH value is 7, the lethality rate of Meningoseptica aureus is greater than 50% after 24 hours, and the lethality rate after 36 hours can be more than 80%;

[0045...

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Abstract

The invention relates to a method for treating regenerated water containing meningoseptic aureus, belonging to the field of water treatment. Add bioflocculant solution to the regenerated water containing Chryseobacterium meningosepticum, add 10 3 A / L lethal plasmid donor bacteria, and adding a concentration of 50-80mg / L nano-scale graphene oxide, the lethality rate of Chryseobacterium meningosepticum is greater than 50% after 24 hours, and the lethality rate can reach 80% after 36 hours above. The advantages are: the biological flocculant derived from the secretion of Bacillus subtilis efficiently flocculates Chryseobacterium meningosepticum and related flora, so as to promote the flocculation and aggregation of bacterial flora cells, and in addition, the low-concentration graphene oxide enhances the conjugation of Chryseobacterium meningosepticum. During the reproductive process, the pNJR6 plasmid spreads efficiently within the flora, so as to achieve the purpose of first flocculation removal and then induction killing to reduce the infection risk of Chryseobacterium meningosepticum in the regeneration water.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a method for inhibiting the infection risk of Meningoseptica aureus in water. Background technique [0002] Reclaimed water is the reuse of sewage after treatment, and is internationally recognized as the "second water source". In the utilization of urban reclaimed water, reclaimed water mainly refers to non-drinking water that can be reused within a certain range after urban sewage or domestic sewage has been treated to a certain water quality standard. According to relevant statistics, 80% of the urban water supply is converted into sewage, and after collection and treatment, 70% of the reclaimed water can be recycled. This means that through the reuse of sewage, the amount of available water in the city can be increased by at least 50% while the existing water supply remains unchanged. Every country in the world attaches great importance to the utilization of recla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/14
CPCC02F1/5263C02F1/56C02F3/34C02F2303/04
Inventor 徐亮孙彩云孙大志庄志军
Owner JILIN INST OF CHEM TECH
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