A method for treating reclaimed water containing Chryseobacterium meningosepticum
A golden bacterium and septic technology, which is applied in the field of inhibiting the infection risk of meningoseptica aureus in water, can solve the problems of population outbreak risk and substandard bacterial population suppression, and achieve the effect of reducing infection risk.
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Embodiment 1
[0029] Include the following steps:
[0030] (a) adding a biological flocculant solution to the regenerated water containing aureus meningoseptica, stirring evenly, the volume ratio of the regenerated water containing aureus meningosepticum to the biological flocculant solution is 90:5, and the biological flocculant solution The concentration of 3g / ml;
[0031] (b) The pNJR6 plasmid was transformed into E. coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as the lethal plasmid donor bacteria;
[0032] (c) adding 10 to the regeneration water of Aureus meningosepticum to which the biological flocculant solution was added in step (a). 3pcs / L lethal plasmid donor bacteria, and adding 50mg / L nanoscale graphene oxide, pH value 7, the lethality rate of meningoseptica aureus was greater than 50% after 24 hours, and the lethality rate after 36 hours was acceptable more than 80%;
[0033] (d) After 60 hours, add a bio...
Embodiment 2
[0035] Include the following steps:
[0036] (a) adding a biological flocculant solution to the regenerated water containing aureus meningoseptica, and stirring evenly, the volume ratio of the regenerated water containing aureus meningosepticum to the biological flocculant solution is 90:8, and the biological flocculant solution is 90:8. The concentration of 4g / ml;
[0037] (b) The pNJR6 plasmid was transformed into E. coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as the lethal plasmid donor bacteria;
[0038] (c) adding 10 to the regeneration water of Aureus meningosepticum to which the biological flocculant solution was added in step (a). 3 pcs / L lethal plasmid donor bacteria, and adding the concentration of 65mg / L nanoscale graphene oxide, the pH value is 7, the lethality rate of meningoseptica aureus is greater than 50% after 24 hours, and the lethality rate after 36 hours can be more than 80%;
[00...
Embodiment 3
[0041] Include the following steps:
[0042] (a) adding a biological flocculant solution to the regenerated water containing aureus meningoseptica, stirring evenly, the volume ratio of the regenerated water containing aureus meningosepticum to the biological flocculant solution is 90:10, and the biological flocculant solution is 90:10. The concentration of 5g / ml;
[0043] (b) The pNJR6 plasmid was transformed into E. coli cells by heat shock transformation method or electroporation method, with a minimum transformation rate of 10%, as the lethal plasmid donor bacteria;
[0044] (c) adding 10 to the regeneration water of Aureus meningosepticum to which the biological flocculant solution was added in step (a). 3 pcs / L lethal plasmid donor bacteria, and adding the concentration of 80mg / L nanoscale graphene oxide, the pH value is 7, the lethality rate of Meningoseptica aureus is greater than 50% after 24 hours, and the lethality rate after 36 hours can be more than 80%;
[0045...
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