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Exposure method for sequencing, sequencing method and device, and storage medium

A technology of exposure device and exposure method, which is applied in the field of sequencing, can solve the problem of excessive exposure time and achieve the effect of accurate sequencing results

Active Publication Date: 2021-06-29
MGI TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides an exposure method for sequencing, a sequencing method and device, and a storage medium, which solve the problem caused by too high or too low exposure time, can better balance the advantages and disadvantages of exposure, and are suitable for long reads Long sequencing ensures that the quality of sequencing will not drop much in the early stage of sequencing, and also leaves sufficient room for the later stage of sequencing to ensure the overall sequencing level and solve the impact of the early stage of sequencing on the later stage of sequencing and the overall sequencing

Method used

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  • Exposure method for sequencing, sequencing method and device, and storage medium
  • Exposure method for sequencing, sequencing method and device, and storage medium
  • Exposure method for sequencing, sequencing method and device, and storage medium

Examples

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Embodiment 1

[0067] This example is based on the sequencer BGISEQ-500RS platform of Huada Genomics; the reagents used come from the library construction kit and single-end sequencing kit used with the sequencer; the verification samples used are from Escherichia coli; The sequencer, reagent operation and other processes used in the process refer to the usage method of the platform; taking SE200 sequencing as an example, the design range of gradient exposure time is 10-140ms.

[0068] In this embodiment, three groups of exposure times are designed for comparison:

[0069] The first group: keep the overall exposure time at 100ms, that is, maintain a constant exposure time.

[0070] The second group: according to exposure time (ms)=A+B·Exp(C·cycle# / N-D), wherein, A=30, B=70, C=3, D=3, N=200, make the exposure time The gradient changes from 34ms to 100ms, which is the exposure time change within the design range.

[0071] The third group: according to exposure time (ms)=A+B·Exp(C·cycle# / N-D)...

Embodiment 2

[0078] This implementation case is based on the sequencer BGISEQ-500RS platform of Huada Genomics; the reagents used are all derived from the library construction kit and paired-end sequencing kit (hereinafter referred to as the PE100 kit) used with the sequencer; the verification samples used It is derived from Escherichia coli; the sequencer, reagent operation and other processes used in the verification process refer to the usage method of this platform; this implementation case takes PE100 sequencing as an example, and the design range of gradient exposure coefficient is 10-130ms.

[0079] In this embodiment, five groups of exposure times are designed for comparison:

[0080] The first group: according to exposure time (ms)=A+B·Exp(C·cycle# / N-D), wherein, A=40.6, B=13.8, C=2, D=2, N=100, make a chain The exposure time was gradually increased from 43ms to 53ms, and the exposure time of the second chain was 50ms for the first part, and 80ms for the latter part.

[0081] The...

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Abstract

An exposure method for sequencing, a sequencing method and device, and a storage medium. The exposure method of the present invention includes, during the image acquisition process, performing exposure in a manner in which the exposure time is gradually increased in the range from 10 ms to 140 ms from the start cycle to the end cycle. The invention solves the problem caused by too high or too low exposure time, can better balance the advantages and disadvantages brought by exposure, is suitable for long-read sequencing, and ensures that the sequencing quality will not drop much in the early stage of sequencing, and also provides Sufficient leeway is left in the later stage of sequencing to ensure the overall sequencing level and solve the impact of the early stage of sequencing on the later stage of sequencing and the overall sequencing.

Description

technical field [0001] The invention relates to the technical field of sequencing, in particular to an exposure method for sequencing, a sequencing method and device, and a storage medium. Background technique [0002] For next-generation sequencing technology, lower cost, higher throughput, and faster speed are its main advantages, but the relatively short read length is the main disadvantage of next-generation sequencing. The speed of next-generation sequencing continues to double, but data processing has become the main bottleneck of next-generation sequencing. Although the short-read sequencing platform can quickly provide huge data fragments, the actual data processing is much more difficult than sequencing to give a definite assembly conclusion. Therefore, long-read length is particularly important, but long-read sequencing is a very big challenge, and it is very difficult to obtain an accurate long-read sequencing result. [0003] During the sequencing process, as t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B30/00H04N5/235
CPCG16B25/00H04N23/70
Inventor 马可心李计广章文蔚刘二凯王静静尹素琴陈奥徐崇钧
Owner MGI TECH CO LTD
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