Marker for early diagnosis and metastasis warning of lung cancer and prepared kit thereof

Abstract

The present invention relates to a marker for early diagnosis and distant metastasis prediction of lung cancer based on platelet LncRNA-STON2 and a prepared kit thereof, and belongs to the fields of medical treatment and public health. The marker is selected from the plasma platelet LncRNA-STON2. The kit prepared from the marker comprises a reverse transcription reaction system, a fluorescence quantitative PCR reaction system and an internal reference system. The present invention also provides a preparation method of the kit. The marker is firstly provided. Compared with healthy donors, an expression level of the LncRNA-STON2 in the plasma platelets of patients with the lung cancer is significantly increased, and a ROC curve analysis shows that AUC is 0.613, sensitivity is 61.5%, and specificity is 59.9%. Compared with the healthy donors, the expression level of the LncRNA-STON2 in patients with early lung cancer is significantly increased, and the ROC curve analysis shows that the AUC is 0.713, the sensitivity is 69.2% and the specificity is 71.9%.

Description

technical field [0001] The invention relates to a platelet LncRNA-STON2-based marker for early lung cancer diagnosis and prediction of distant metastasis and a kit for its preparation, belonging to the field of medical and health care. Background technique [0002] Lung cancer is a malignant tumor with the highest mortality and morbidity in all countries in the world and in my country. Its onset is hidden, and its early clinical manifestations lack specificity. Most patients are already in locally advanced stage or distant metastasis when they are diagnosed, and the prognosis is often poor.5 The annual survival rate is only 2%. Therefore, there is an urgent need for a more sensitive diagnostic marker to provide an objective basis for early diagnosis, early treatment and prognosis of lung cancer with distant metastasis. [0003] Platelets are anuclear blood cells with a diameter of 2-4 μm, derived from megakaryocytes in the bone marrow, with a short half-life of only 7-10 day...

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Problems solved by technology

[0004] Existing clinical diagnostic techniques such as X-ray and B-ultrasound cannot be detected in the early clinical stage, while CT, MRI and PET examinations can find smaller tumor tissues, but their specificity is low, and pathological biopsy is still needed to diagnose

The use of endoscopes such as fiberoptic bronchoscopes can improve the diagnosis rate of non-small cell lung cancer, but they are all interventional examinations, which cause great damage and pain to patients, and require high technical skills for operators, and not all parts of the lungs Biopsy can be taken, not suitable for large-scale population screening and census

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