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Chronic obstructive pulmonary disease detection kit

A chronic obstructive pulmonary and disease detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as time-consuming, expensive, and indistinguishable, and achieve good market prospects

Active Publication Date: 2019-07-23
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the diagnosis of COPD mainly relies on pulmonary function tests, chest X-ray examinations, chest CT examinations, etc. However, pulmonary function tests (spirometry) are time-consuming and expensive procedures that can only be performed by specialist pulmonary physicians
However, the above methods cannot be effectively distinguished from other diseases such as asthma, bronchitis, pulmonary fibrosis, tuberculosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Analysis of the association between Thrombospondin-2 protein and COPD

[0028] 1. Method

[0029] 1.1 Subject

[0030] Include 80 healthy controls, 126 COPD patients, and 146 AECOPD patients. The subject population had been informed and agreed before the experiment.

[0031] 1.2 Lung function test

[0032] Check steps:

[0033] (1) Ask the medical history, and explain to the patient the purpose of the examination, examination process and precautions before the operation;

[0034] (2) Enter basic patient information;

[0035] (3) Clamp the patient's nose with turbinates and instruct the patient to keep breathing through the mouth;

[0036] (4) Keep the disposable mouth as tight as possible to ensure no air leakage during the test;

[0037] (5) According to the inspection items, guide the patient to cooperate with the immediate exhalation and inhalation through the password;

[0038] (6) Collect data, analyze and print reports;

[0039] Meaning of main indicators:

[0040] (1) F...

Embodiment 2

[0063] Example 2 The kit of the present invention

[0064] 1. Composition of the kit of the present invention

[0065]

[0066] 2. How to use the kit

[0067] (1) Add 50μL of standard or sample to each well of 96-well plate, after sealing the membrane, incubate with shaking at room temperature for 2 hours;

[0068] (3) Wash the plate 3 times with 100μL plate washing solution;

[0069] (4) Add 50μL of biotin-labeled secondary antibody to each well, and incubate with shaking at room temperature for 30min after sealing the membrane;

[0070] (5) Wash the plate 3 times with 100μL plate washing solution;

[0071] (6) Add 50μL of chromogenic substrate to each well, shake and mix at room temperature for 2 minutes;

[0072] (7) Reading of microplate reader;

[0073] In summary, the present invention can realize effective detection of COPD by detecting Thrombospondin-2protein in blood.

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Abstract

The invention relates to the field of in vitro diagnosis of pulmonary disease, in particular to a chronic obstructive pulmonary disease detection kit. According to the chronic obstructive pulmonary disease detection kit, the content of Thrombospondin-2 in blood is detected, and thus diagnosis of chronic obstructive pulmonary disease is achieved. The chronic obstructive pulmonary disease detectionkit is simple and effective, and has good application prospects.

Description

Technical field [0001] The invention relates to the field of in vitro diagnosis of lung diseases, in particular to a detection kit for chronic obstructive pulmonary disease. Background technique [0002] Chronic obstructive pulmonary disease (COPD) is a kind of chronic bronchitis and / or emphysema with airflow obstruction characteristics, which can further develop into common chronic diseases of pulmonary heart disease and respiratory failure. It is related to the abnormal inflammatory reaction of harmful gases and harmful particles, and the disability rate and fatality rate are very high. The global incidence rate of over 40 years old has reached 9% to 10%. [0003] At present, the diagnosis of COPD mainly relies on pulmonary function examination, chest X-ray examination, chest CT examination, etc. However, pulmonary function test (spirometry) is a time-consuming and expensive procedure that can only be performed by specialist pulmonary physicians. However, the above methods canno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/558G01N33/68
CPCG01N33/50G01N33/558G01N33/6812
Inventor 王浩申永春李晓欧文富强
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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