Gene sequencing chip, equipment and manufacturing method

A technology of gene sequencing and manufacturing method, which is applied in the field of gene sequencing chip manufacturing, gene sequencing chip, and gene sequencing equipment, and can solve problems such as crosstalk of gene sequencing chips

Pending Publication Date: 2019-07-30
BOE TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention at least partly solves the problem of crosstalk in the existing gene sequencing chip, and provides a method for manufacturing a gene sequencing chip, a gene sequencing device, and a gene sequencing chip

Method used

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  • Gene sequencing chip, equipment and manufacturing method
  • Gene sequencing chip, equipment and manufacturing method
  • Gene sequencing chip, equipment and manufacturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] see Figure 2a and Figure 3a , this embodiment provides a gene sequencing chip, including a substrate 1 and a plurality of microporous structures 2 arranged on the substrate 1, and a light blocking structure 3 is arranged between adjacent microporous structures 2, and the light blocking structure 3 is used for The light emitted from the bottom of the microhole structure 2 is blocked from going to the detection unit 5 corresponding to the adjacent light blocking structure 3 .

[0032] The material of the substrate 1 is, for example, glass, silicon, or organic resin materials. The material of the base 1 of organic resin is, for example, polymethyl methacrylate (PMMA).

[0033] Such as Figure 2a As shown, the boundary of the microporous structure 2 may be formed on the substrate 1 and the light blocking structure 3 and the surface modification layer 4 on the substrate 1 . another example Figure 3a As shown, the boundaries of the microporous structure 2 are formed o...

Embodiment 2

[0050] This embodiment provides a gene sequencing device, including the gene sequencing chip of embodiment 1.

[0051] That is to say, the gene sequencing chip provided in Example 1 is applied to a gene sequencing device. Figure 2b and Figure 3b Only the detection unit 5 in the gene sequencing device is shown in . The parts except the gene sequencing chip can be configured according to the existing technology, so details will not be described here.

[0052] The gene sequencing equipment using the gene sequencing chip can effectively suppress or avoid the problem of crosstalk.

Embodiment 3

[0054] This embodiment provides a method for manufacturing a gene sequencing chip, comprising: a step of forming a plurality of microporous structures 2 on a substrate 1; a step of forming a light blocking structure 3 between adjacent microporous structures 2, the light blocking structure 3 is used to block the light emitted from the bottom of the microhole structure 2 from going to the detection unit 5 corresponding to the adjacent light blocking structure 3 . The gene sequencing chip prepared in this way can effectively avoid the problem of crosstalk.

[0055] Optionally, a step of forming a surface modification layer 4 covering the light blocking structure 3 is also included, wherein the surface modification layer 4 is used for receiving surface modification during gene sequencing. In this way, the reagent carrying the DNA molecule 7 can be combined with the surface modification layer 4 in subsequent applications to complete gene sequencing.

[0056] Two examples of specif...

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PUM

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Abstract

The invention provides a gene sequencing chip, equipment and a manufacturing method, and belongs to the technical field of gene sequencing. The problem that for an existing gene sequencing chip, crosstalk exists in application can be at least partially solved. The gene sequencing chip comprises a base and multiple micro-porous structures arranged on the base, and light-blocking structures are arranged between the adjacent micro-porous structures and used for blocking light emitted from the hole bottoms of the micro-porous structure from shining to detection units corresponding to the adjacentlight-blocking structures.

Description

technical field [0001] The invention belongs to the technical field of gene sequencing, and in particular relates to a gene sequencing chip, a gene sequencing device, and a method for manufacturing the gene sequencing chip. Background technique [0002] Gene sequencing refers to the analysis of the sequence of bases in a specific gene (DNA molecule with a certain length) by physical, chemical, and biological means. There are only four bases in DNA, adenine (A), thymine (T), and cytosine (C). [0003] and guanine (G). Since the first-generation Sanger sequencing in 1977, gene sequencing technology has developed for three generations, including the second-generation high-throughput sequencing technology represented by Illumina, and the third-generation single-molecule sequencing technology dominated by Pacific Biosciences. Although different generations of sequencing technologies have their own characteristics, in terms of sequencing principles, all sequencing is based on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/6869C12Q2565/631
Inventor 张笑谷新
Owner BOE TECH GRP CO LTD
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