Kit and using method thereof
A technology of kits and primer pairs, which is applied in biochemical equipment and methods, DNA/RNA fragments, determination/inspection of microorganisms, etc., and can solve problems such as poor reaction conditions, low amplification efficiency, and poor typing effect.
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[0013] One aspect of the present invention relates to a kit, comprising a kit for amplifying STR loci D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, vWA, Penta E, PentaD and Amelogenin One or more of the 16 pairs of upstream and downstream primers, the sequences of the 16 pairs of upstream and downstream primers are shown in SEQ ID NO: 1-32.
[0014] The kit of the present invention involves 16 pairs of upstream and downstream primers. The above 16 pairs of upstream and downstream primers have the advantages of good specificity and high amplification efficiency. Specific amplification and cross-reaction avoid the impact of non-target products on the typing results. At the same time, the amplification efficiency is high and the detection signal is strong. On the one hand, it can shorten the PCR amplification time. On the other hand, due to the high detection signal, it can be quickly Accurate detection of PCR products and STR typing...
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