Use of glaucocalyxin A in preparation of medicament for treating liver cancer
A technology of Wangzaozi B and treatment of liver cancer, applied in the fields of biochemistry and molecular biology, can solve the problems of large side effects and damage to the human body's own immunity
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[0025] Example: Extraction and Verification of Wangzaozi B
[0026] Refer to CN105585471A to extract and verify Wangzaozi B. Briefly, the extraction steps are as follows: (1) The root of Wangzaozi is crushed with an ordinary pulverizer, and passed through a 40-mesh sieve to obtain the coarse powder of Wangzaozi root; (2) Accurately weigh the root of Wangzaozi Coarse powder 5.0kg, add 60gNaHCO 3 Mix the additives evenly, grind them in a super mill for 40 minutes, pass through a 300-mesh sieve, accurately weigh 2.0 kg and put them in a beaker, add 80% ethanol aqueous solution according to the ratio of material to liquid 1:24, and stir evenly; (3 ) was ultrasonically extracted at 45°C, frequency 43kHz, and power 250W for 50 minutes, and the extract was vacuum filtered to obtain a filtrate, which was concentrated under reduced pressure at 40°C and 60r / min to obtain a concentrate; (4) the concentrate was sequentially Through petroleum ether and chloroform extraction; (5) take the ...
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